Repeated production of lxylulose by an immobilized whole英文资料.pdfVIP

Biochemical Engineering Journal 96 (2015) 23–28 Contents lists available at ScienceDirect Biochemical Engineering Journal j o u r n a l h o m e p a ge : w w w. elsev /locate/bej Repeated production of l-xylulose by an immobilized whole-cell biocatalyst harboring l-arabinitol dehydrogenase coupled with an NAD+ regeneration system Hui Gao a , In-Won Kim a , Joon-Ho Choi b , Eshita Khera c , Fei Wen d,∗∗ , Jung-Kul Lee a,∗ a Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea b Department of Food Science and Biotechnology, Wonkwang University, Iksan-city, JeonBuk, 570-749 Republic of Korea c Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA d Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA a r t i c l e i n f o a b s t r a c t Article history: The biotransformation of l-arabinitol into l-xylulose was investigated using whole-cell biocatalysts. Received 20 October 2014 Efficient production of l-xylulose was accomplished using Escherichia coli that expressed l-arabinitol Received in revised form dehydrogenase (LAD). The production yield was enhanced by coupling LAD with a cofactor-regenerating 22 December 2014 NADH oxidase in an E. coli whole-cell biocatalyst system. Factors affecting the production of l-xylulose by Accepted 26 December 2014 this whole-cell biocatalyst system include reaction pH, maximal cell loading, and cofactor