麝香保心丸、脉平对腹主动脉狭窄高血压大鼠心肌肥厚的作用.docVIP

精品论文 参考文献 麝香保心丸、脉平对腹主动脉狭窄高血压大鼠心肌肥厚的作用 柳玲1 石磊2 黄杲1 梁绪国2(通讯作者) （1 山东省烟台白石肛肠医院 山东烟台 264001） （2 山东省烟台毓璜顶医院 山东烟台 264000） 【摘要】目的 在腹主动脉狭窄高血压大鼠模型上观察了心肌肥厚的变化，并探讨其与多种细胞因子、基质金属蛋白酶、心肌细胞凋亡率、凋亡调控基因表达等多种因素之间的关系及中药麝香保心丸、脉平对心肌肥厚和各种影响因素的作用。方法　双抗夹心ELISA法测定MMP-2、MMP-9和IL-6、IL-10；放免法检测TNF-alpha;；流式细胞法测心肌细胞凋亡率和凋亡调控基因表达。结果　腹主动脉狭窄6周后，大鼠左室重及室重/体重比值明显增加；同时多种细胞因子、基质金属蛋白酶、心肌细胞凋亡率、凋亡调控基因表达等均发生了相应的变化；麝香保心丸和脉平对心肌肥厚和各种影响因素有较好的治疗和预防作用。结论　心肌肥厚可能与多种因素有关；麝香保心丸和脉平对预防和治疗心肌肥厚有一定的作用。 【关键词】 心肌肥厚 麝香保心丸 脉平 细胞因子 基质金属蛋白酶 凋亡 【中图分类号】R965 【文献标识码】A 【文章编号】2095-1752（2013）28-0151-02 Effect of Shexiangbaoxinwan and Maiping on Left Ventricle in Pressure-Overloaded Hypertrophy Rat 【ABSTRACT】 AIM：To explore the effect of Shexiangbaoxinwan and Maiping on left ventricle in pressure-overloaded hypertrophy rats. And also the relationship between left ventricular hypertrophy (LVH) and cytokines, matrix metalloproteinases, apoptosis, and apoptosis genes. METHODS: IL-6, IL-10, MMP-2, MMP-9 were evaluated by ELISA means. TNF-alpha was measured with radioimmunoassays. Flow cytometer was used to measure the apoptosis ratio and the expression of Fas , Bcl-2 and Bax. RESULTS: 6 weeks after abdominal aortic coarctation operation, compared with the sham operated group, the weight of the left ventricle (0.995plusmn;0.320g) and heart weight/body weight (0.369plusmn;0.149) were significantly increased in modern group. And also IL-6 (213.56plusmn;83.41pg/ml), TNF-alpha;(1.997plusmn;0.812ng/ml), MMP-2 (72.91plusmn;35.59), MMP-9 (60.15plusmn;26.43), and apoptosis genes (Fas, Bax, Bcl-2, Bcl-2/Bax) expression were increased；apoptosis ratio (9.81plusmn;5.03), IL-10 (91.07plusmn;38.74pg/ml) decreased compared with sham operated group. The 2 groups with drugs (SB,MP) decreased the increasing weight of LV and ventricular weight/body weight; and inhibited the changes of influencing factors (IL-6, IL-10, TNF-alpha;, MMP-2, MMP-9, apoptosis ratio, apoptosis genes expression). CO