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Chapter 19 Overview of Nucleic Acid Structure Chapter 18 Metabolism of Nucleotides 3. Complete the Biosynthesis of UTP and CTP (eq 18.3; Fig 18.12) 2. Regulation and Specificity of RR (Table 18.1) Fig 18.17 Human dihydrofolate reductase (DHFR) (Fig. 18.17) Purine breakdown gives uric acid (Fig 18.22) Animated View of Nucleic Acid Synthesis 4. Okazaki Fragment Processing (Fig 20.13) G. Termination Requires TUSS Protein (Fig 20.17) H. DNA Replication in Eukaryotes I. DNA Repair 3. Direct Repair of Thymine Dimers (Figs 20.21, 20.22) 4. Overview of Excision Repair (Fig 20.23) 5. Excision Repair of Deaminated Cytosine (Figs 20.24, 20.25) Excision Repair of Deaminated Cytosine (continued) J. Recombination The Holiday Model of Homologous Recombination Fig 20.27) A. The Central Dogma of Information Flow (modified Fig 21-1) B. All RNA types are transcribed from DNA C. The Prokaryote Operon Promoter Region (Fig. 21-6) D. RNA Polymerase. 1) Holoenyme composition 2. The crystal structure shows “Open Hand” architecture (Fig 21.2). 3. Mechanism of RNA polymerization (Fig 21.3). 4. Some transcription terminology (Fig. 21-5) E. Transcription Initiation 1. Role of the sigma subunit (Fig 21.7) Initiation (Fig 21.7), continued. 2. Variety of sigma subunits in E. coli (Table 21-3) F. Transcription Elongation: Bubble Movement G. Transcription Termination 1. Termination sequences; Hairpin formation in the transcript (Fig 21.8) 2. Rho termination (Fig 21.9) H. Eukaryotic Transcription: 1) RNA polymerases 2. The “Open Hand” architecture in eukaryote RNA pol (Fig 21.10) 3. Eukaryote transcription factors 4. The initiation complex I. Transcription Regulation in Prokaryotes. 1. Some Definitions Definitions continued 3. Four regulation paradigms 3. continued 3. continued 3. continued J. The lac Operon, an Example of Negative Regulation J. The Lac Operon: an Example of Negative Regulation: 1. Proteins of the Lac operon 2. Organization of the genes for prote
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