黑鲷与真鲷及其杂交子一代脂肪含量测定与脂肪酸成分分析-食品科学.pdf

※成分分析 食品科学 2016, Vol.37, No.20 81 黑鲷与真鲷及其杂交子一代脂肪含量测定与 脂肪酸成分分析 1 1 2, * 2 1 2 2 2 王思婷 ，李 鹏 ，陈淑吟 ，张志勇 ，尹绍武 ，祝 斐 ，贾超峰 ，刘海林 （1.南京师范大学生命科学学院，江苏省生物多样性与生物技术重点实验室，江苏 南京 210023 ； 2.江苏省海洋水产研究所，江苏 南通 226007 ） 摘  要：为了解真鲷、黑鲷及杂交子代的脂肪和脂肪酸含量与成分，采用索氏抽提法分析4 种鲷鱼肝脏、肌肉和鱼 骨的脂肪含量，气相色谱法分析脂肪酸组成。结果表明，正、反杂交子代均良好地继承了亲本真鲷的优势，脂肪含 量明显高于亲本黑鲷，且以肝脏中最为显著。杂交后代与亲代的饱和脂肪酸成分组成情况基本吻合，杂交后代中产 生了C12 0 、C22 0 、C24 0等几种亲代脂肪酸成分中未检测到的脂肪酸，说明杂交后代脂肪酸组成更丰富。杂交后代的 ∶ ∶ ∶ 不饱和脂肪酸组成与黑鲷亲本基本相同，尤其体现在肝脏组成上。 关键词：真鲷；黑鲷；杂交育种；脂肪；脂肪酸 Lipid Content and Fatty Acid Profile of Acanthopagrus schlegeli , Pagrosomus major and Their Hybrids 1 1 2, * 2 1 2 2 2 WANG Siting , LI Peng , CHEN Shuyin , ZHANG Zhiyong , YIN Shaowu , ZHU Fei , JIA Chaofeng , LIU Hailin (1. Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China; 2. Institute of Oceanology and Marine Fisheries, Nantong 226007, China) Abstract: The lipid contents of the liver, muscles and bones of Pagrosomus major , Acanthopagrus schlegeli and their hybrids were analyzed by Soxhlet extraction method, and the fatty acid composition was tested by gas chromatography. The results showed that the hybrids fully inherited the advantages of the red sea bream parents and their lipid content was significantly higher than that of the parent black porgy, and the most significant difference in lipid content was observed for  liver among the three fish tissues. The saturated fa