医学论文-用阿托伐他汀保护急性脑缺血大鼠脑的研究.docVIP

医学论文-用阿托伐他汀保护急性脑缺血大鼠脑的研究 ????????????? 作者：陈凤，王江昆，王德贵，侯一平，宋焱峰 【摘要】? 目的：探讨阿托伐他汀对急性脑缺血大鼠的脑保护作用. 方法：健康Wistar大鼠85只，雌雄不限,分为假手术组、手术组、阿托伐他汀组. 给药量分别为 3,8,10 mg/kg，连续灌胃14 d,于末次给药后1 h采用改良的线栓法制备大鼠大脑中动脉永久性缺血模型(pMCAO)，制模成功后8 h观测各组的神经行为变化,之后处死进行红四氮唑染色观察脑梗塞体积,HE染色观察脑组织形态结构变化，免疫组织化学方法检测CD34的表达并测量微血管计数(MVC)，同时检测血清总胆固醇含量. 结果：阿托伐他汀给药组(3 mg/kg)大鼠脑缺血损伤程度减轻(P0.01)、梗死灶面积减少(P0.01),CD34阳性细胞表达增高(P0.05)，微血管计数增多(P0.05)，血清总胆固醇含量无统计学差异(P0.05). 结论：低剂量阿托伐他汀(3 mg/kg)对急性脑缺血大鼠即具有脑保护作用. 【关键词】? 阿托伐他汀；脑缺血；药物疗法；红四氮唑；CD34；微血管计数 　　【Abstract】 AIM: To investigate the protective effects of atorvastatin on acute cerebral ischemia injury in rats. METHODS: Eightyfive healthy Wistar rats (female and male) were randomly divided into 5 groups: sham operated group, ischemic group, and atorvastatin 3, 8, 10 mg/kg groups. Rats were fed atorvastatin for 14 days successively. One hour after the last administered atorvastatin the modified way was used to prepare permanent middle cerebral artery occlusion (pMCAO) model. After 8 h of cerebral ischemia injury, the extent of neurological deficits was evaluated. The infarct area was measured by TTC staining technique. HE staining was used to observe brain tissue changes, the immunohistochemical method was used to examine CD34 expression and microvessel count (MVC).The blood serum total cholesterol content was also examined. RESULTS: In 3 mg/kg atorvastatin group the infarct size and neurological deficit scores distinctly decreased (P0.01), CD34 positive cells distinctly increased (P0.05), MCV also increased (P0.05), but the blood serum total cholesterol content had no significant change (P0.05). CONCLUSION: Low dose atorvastatin has protective effects against focal cerebral ischemia in rats. 　　【Keywords】 atorvastatin; brain ischemia/cerebral; drug therapy; TTC; CD34; microvessel count 　　0? 引言??? 　　脑血管疾病是目前严重危害人类健康的主要疾病之一，随着当今社会的老龄化，脑血管病发病率不断增高. 在联合国世界卫生组织调查的57个国家中死于脑血管病者占11.3%, 其中40个国家脑血管病占死因前三位［1］. 近年来对阿托伐他汀的非降脂作用受到关注，研究已发现中风后给予阿托伐他汀治疗可以促进大脑的可塑性，增强神经功能的恢复，促进新生血管的生成［2］.