医学论文-甲醛对小鼠生精细胞Bcl-2、Bax蛋白表达及超微结构改变的影响.docVIP

医学论文-甲醛对小鼠生精细胞Bcl-2、Bax蛋白表达及超微结构改变的影响 【摘要】? 目的:研究甲醛对小鼠睾丸生精细胞Bcl-2、Bax蛋白表达及超微结构的改变。方法:取24只雄性清洁级（ICR）小鼠，随机分为4组，每组6只，用0.2、2、20 mg/kg甲醛腹腔注射，每天一次，连续5 d，第6天用免疫组化法测定小鼠睾丸生精细胞的Bcl-2、Bax蛋白表达率；用电镜观察小鼠睾丸生精细胞超微结构变化。结果:小鼠睾丸生精细胞的Bcl-2蛋白表达率明显低于对照组，差异有显著性（P＜0.05），Bax蛋白表达率明显高于对照组，差异有显著性（P＜0.05）；电镜观察结果显示，各浓度甲醛能使生精细胞的细胞核、染色质、线粒体等超微结构发生不同程度的病理改变。结论:甲醛可使小鼠生精细胞Bcl-2和Bax蛋白表达及超微结构发生显著性改变，从而诱导细胞凋亡。 【关键词】? 甲醛 Bcl-2 Bax 生精细胞 ??? Abstract:? Objective:To study the effects of formaldehyde on Bcl-2, Bax expression in spermatogenic cell of mice and ultrastructural changes in spermatogenic cell. Methods: Twenty-four male mice (ICR) were divided into 4 groups randomly, who were treated with formaldehyde of 0.2、2、20 mg/kg respectively one time a day, and lasted for 5 days, the Bcl-2, Bax protein expression rate was tested in the sixth day with the immunohistochemical method; and the ultrastructural changes of spermatogenic cell and sperm were observed under the transmission electron microscope. Results: Bcl-2 protein expression rates were lower than that of the contrast group, the difference had statistical significance（P0.05）. Bax protein expression rate was higher than that of the contrast group, the difference had statistical significance（P0.05）. The result under electron microscope showed that the ultrastructure of karyon, karyotin, mitochondria and karyotheca in each treated group had different degree of damage by serving formaldehyde in different concentrations.Conclusion: Formaldehyde exposure will cause the remarkable changes of Bcl-2, Bax protein expression, and ultrastructural changes in spermatogenic cell and sperm, which led to cellular apoptosis. ??? Key words:? formaldehyde, Bcl-2, Bax, spermatogenic cell ??? 动物实验发现,甲醛具有生殖毒性和致细胞突变及致癌性[1]。对甲醛毒性研究一般集中在器官和组织水平，从分子水平探讨甲醛对机体损伤及细胞凋亡的研究国内较少。为了进一步研究甲醛睾丸细胞毒性和生殖细胞的调亡机制，本研究分析Bcl-2、Bax蛋白表达及小鼠睾丸生精细胞超微结构变化情况,报告如下。 ??? 1? 材料和方法 ??? 1.1? 主要试剂? AR甲醛（Formaldehyde，FA），由上海溶剂厂提供，Bal-2、Bax免疫组化试剂盒（购自武汉博士德生物制剂有限公司），戊二醛（购自中国医药集团上海化学试剂公司）环氧树脂（购自Epon812，Serva公司）。