医学论文-吸入汽油废气颗粒大鼠再行卵蛋白攻击后哮喘速发相反应变化.docVIP

医学论文-吸入汽油废气颗粒大鼠再行卵蛋白攻击后哮喘速发相反应变化 ?????????? 作者：元熙哲，李今子，金正勇，池永学，尹永日 【摘要】? ［目的］观察吸入不同时间长度汽油废气颗粒（DEP）的大鼠行卵蛋白攻击后哮喘速发相反应的变化.［方法］选择雄性Wistar大鼠，随机分为6个组，其中A组给予生理盐水攻击，B组给予卵蛋白攻击，C，D，E，F组分别给吸入DEP各1，2，3，4周后再行卵蛋白攻击.抗原攻击结束1周后除A组外的其他各组大鼠给予卵蛋白激发30min，测量气道阻力5min.观察肺泡灌洗液中炎症细胞及血清总IgE值的变化，检测肺组织中IL5和IFNγ水平.［结果］抗原激发后B，C，D，E，F组大鼠气道阻力均高于A组，E，F组明显高于B组；气道阻力的增高程度和DEP的吸入时间呈正相关.除A组外的各组大鼠肺泡灌洗液中均可观察到大量炎症细胞浸润，包括嗜酸性粒细胞、中性粒细胞、淋巴细胞和巨噬细胞，以???两者为主.嗜酸性粒细胞体积分数在B组与A组之间及E，F组与B组之间差异均有统计学意义；中性粒细胞体积分数在A，B，C，D组之间无显著性差异，但E，F组较A，B组显著增高.B，C，D，E，F组大鼠血清总IgE值较A组显著增高，F组与B组间差异亦有统计学意义；D，E，F组大鼠肺组织中IL5水平较A，B组显著增高.［结论］吸入DEP大鼠再行卵蛋白攻击后哮喘速发相反应明显增强，反应增强程度与DEP吸入时间呈正相关. 【关键词】? 哮喘；车辆排放物；卵蛋白质类；大鼠 ??? ABSTRACT:OBJECTIVETo observe the change of early reaction of the rat resensitised by ovalbumin after preinhaled diesel exhaust particulates(DEP).METHODSMale rats were randomly divided into 6 groups.? Group A was sensitizated with saline and group B was sensitizated with ovalbumin. The groups C，D，E and F were inhaled with DEP for 1 week, 2 weeks, 3 weeks and 4 weeks, respectively, and after DEP inhalation once more sensitizated with ovalbumin. After 1week of end of antigen sensitization, except group A another groups were challenged with ovalbumin 30 minits，and observe change of lung function for 5 minits. Finally observe change of inflammation cell in alveolus lavage fluid, serum overall IgE, concentra tion of IL5 and IFNγ? in lung tissue.RESULTSThe airway resistance was more increased in group B, C, D, E and F than in group A, and group E and F was more increased than group B after antigen challenge. The degree of airway hyperresponsiveness depended on the inhalation time. There were many infiltration of inflammatory cells except group A, including eosinophil, neutrophil, lymphocyte and macrophase, eosinophil and neutrophil were major. Eosinophils increased in group B than in group A, in group E and F than in group B, and the difference was a significant. Neutrophils were no significant difference among group A, B, C, D, but mo