医学论文-体外消化透析法测定朱砂中汞生物可接受率的实验条件确定.docVIP

医学论文-体外消化透析法测定朱砂中汞生物可接受率的实验条件确定 ???????????????????作者：庞京团 胡广林 韩彬 齐江宁 李平 【摘要】? 目的确定体外消化透析法测定朱砂中汞的生物可接受率的实验条件，建立实验流程，并测定朱砂中汞的生物可接受率。方法模拟胃肠消化环境，研究pH、接触时间等因素对朱砂中汞的溶出率及透析时间等对朱砂中汞的生物可接受率的影响规律，确定合适模拟条件。结果确定的实验流程模拟条件为：液/固比100，人工胃液pH=1.5，加入0.16%胃蛋白酶1 ml，接触时间100 min，透析时间100 min，加入胰液胆盐混合物量为5 ml。在确定的实验条件下，CV-AFS测得水飞朱砂中汞的溶出率为2.5×10-6（n=6，RSD＝4.1％），生物可接受率为4.8×10-7 (n=6，RSD＝3.5％)。结论体外消化透析法可用于测定朱砂中汞的生物可接受率，可为测定含朱砂中成药中汞的生物可接受率提供方法学上的参考。 【关键词】? 体外消化透析法； 朱砂； 生物可接受率； 汞 　　Abstract：ObjectiveTo determine the simulated conditions for estimating the bioaccessibility of mercury from cinnabar by means of an in vitro digestion and dialysis.MethodsIn order to determine the appropriate simulated conditions, we simulated gastrointestinal digestion environment, and studied the effects of contact time,the pH of artificial gastric juice, addition of pepsin, dialysis time, addition of pancreatin bile salt mixture.ResultsThe simulated conditions were determined as follows: liquid/solid of 100, the pH value of 1.5 in artificial gastric juice, 1 ml pepsin which contained 0.16 g pepsin per ml, contact time of 100 min, 5 ml pancreatin bile salt mixture which contained 0.004 g pancreatin and 0.025 bile salt per ml,dialysis time of 100 min. We estimated the bioaccessibility of mercury from cinnabar by CV-AFS, and the mercury dissolution rate and bioaccessibility from cinnabar showed high repeatability, dissolution rate was about 2.5×10-6(n=6,RSD＝4.1％), and the bioaccessibility was about 4.8×10-7(n=6,RSD＝3.5％).ConclusionThe in vitro digestion and dialysis are suitable for estimating the bioaccessibility of mercury from Chinese traditional patent medicine which contains cinnabar. 　　Key words：In vitro digestion and dialysis；? Cinnabar；? Bioaccessibility；? Mercury??? 　　朱砂又名丹砂、辰砂等，始载于《神农本草经》，列为上品药，主含硫化汞(HgS)；其性微寒，有毒，归心经，有镇心安神、清热解毒之功，主治心神不宁、心悸消渴等病症［1］ 。近年来关于朱砂及含朱砂中成药对肾脏、中枢神经系统及生殖系统的毒性屡见报道［2］ 。目前，国际上对中药中重金属的毒副作用已相当重视，FDA和WHO均制定了类似食品、蔬菜以及茶叶等产品的重金属允许摄入量。美国、欧盟、日本等国家及我国传统中药出口的东南亚地区都制定了严格的重金属限量标准。但是，包括我国在内，所有针对汞制定的限