近江牡蛎等7种养殖鱼虾贝类参照基因β-肌动蛋白cDNA序列的克隆与比较分析 Cloning and Analysis of the Beta-Actin Gene in Jinjiang Oyster (Crassostrea ariakensis) and Other Six Cultivated Aquatic Animals.pdfVIP
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近江牡蛎等7种养殖鱼虾贝类参照基因β-肌动蛋白cDNA序列的克隆与比较分析 Cloning and Analysis of the Beta-Actin Gene in Jinjiang Oyster (Crassostrea ariakensis) and Other Six Cultivated Aquatic Animals
2008正第3卷 生 态 毒 理 学 报 V01.3,2008
第3期, 256—261 AsianJournalofEcotoxicology
近江牡蛎等7种养殖鱼虾贝类参照基因13l-肌动蛋白
eDNA序列的克隆与比较分析
林群,梁旭方4,王琳,李光照,胡永乐
暨南大学生命科学技术学院,广州510632
螺(Cipangopaludinacahayensis)、日本沼虾(Macrobrachiumnipponensis)、斑鳢(Channa
nobilis)、鲮鱼(Cirrhinus
鳙鱼(Aristichthys
基酸序列.所获基因与其它动物类群的B.肌动蛋白基因氨基酸同源性高达96%以上,表明软体类、节肢类、鱼类、两栖
类、鸟类和哺乳类等不同类群动物D.肌动蛋白基因在生物进化过程中高度保守.系统发育分析显示软体类、节肢类、鱼
类、两栖类、鸟类和哺乳类脊椎动物B一肌动蛋白分类与传统的分类基本一致.
关键词:B.肌动蛋白基因;基因克隆;序列分析;近江牡蛎(Crassostreaariakensis)
文章编号:1673.5897(2008)3.256.06中图分类号:Q78 文献标识码:A
and oftheBeta·Actinin
CloningAnalysis Gene Jinjiang
Six
OtherCultivatedAnimals
(Crassostreaariakensis)and Aquatic
LIN
Qun,LIANG Lin,LI
Xu.fang+,WANGGuang.zhao,HUYong.1e
ofLifeScienceand 510632
CoUege Technology,JinanUniversity,Guangzhou
Received26December2007 5March2008
accepted
Abstract:Inorderto the and offunctionalin cDNAofbeta.actin
studyexpressionregulation genesaquaticanimals.full—length gene
in liverof
the obtainedbvRT-PCRandRACEmethods.Resultsshowedthatthe
Jinjiangoyster(Crassostreaariakensis)was
beta-actincDNAin was in an frameof1 376
complete Jinjiangoyster 1343bplength,containingopenreading
amino 5’UrRand 3’UTR.PartialcDNA of
acids),flankedby68bp 144bp sequences
shrimp(Macrobrachiumnipponensis),Taiwansnakehead(Channamaculata),catfish(Clariasfuscus),bighead
mud werealSO revealedthattheidentitiesof
nobilis)and molitorella)beta.aedn
carp(Cirrhinus gene obtained.Sequenceanalysis
beta-acf
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