白藜芦醇对重症急性胰腺炎大鼠脑损伤的保护作用.docVIP

白藜芦醇对重症急性胰腺炎大鼠脑损伤的保护作用 【摘要】 目的 探讨白藜芦醇对实验性重症急性胰腺炎(SAP) 大鼠脑损伤的的保护作用。方法 96只成年雄性SD大鼠随机分为假手术(SO)组;重症急性胰腺炎(SAP)组;地塞米松(DEX)治疗组和白藜芦醇(RES)治疗组。各组大鼠均于制模后3、6、12h采集标本。酶联免疫吸附试验(ELISA)测定血清髓鞘碱性蛋白(MBP)、细胞间紧密连接蛋白(Zo1)、TNFα和IL6含量;透射电镜观察胰腺和脑组织超微结构的变化。结果 SAP组各时间点MBP和Zo1水平均明显高于SO组(Plt;0.05)，RES组和DEX组MBP和Zo1水平较SAP组明显降低(Plt;0.05)。RES组和DEX组TNFα、IL6含量均明显低于SAP组(Plt;0.05)。SAP组出现明显神经元细胞肿胀、毛细血管淤血、血栓形成、线粒体肿胀和细胞凋亡等超微结构的变化，RES组和DEX组上述改变明显减轻。RES组各项指标与DEX组差异不显著(Pgt;0.05)。结论 Zo1的降解参与了大鼠SAP脑损伤的病理过程;MBP可以作为SAP脑损伤程度的判断指标;白藜芦醇可以降低SAP大鼠脑血管上皮损伤程度，从而起到减轻SAP脑损伤的作用。 【关键词】 重症急性胰腺炎;白藜芦醇;脑损伤 　　ABSTRACT: Objective To explore the protective effect of resveratrol on rat brain injury resulting from severe acute pancreatitis (SAP). Methods Ninetysix male SpragueDawley rats were randomly divided into four groups: shamoperation (SO) group, severe acute pancreatitis (SAP) group, resveratroltreated (RES) group and dexamethasonetreated (DEX) group, with eight rats in each group evaluated at 3, 6 and 12h. Levels of serum myelin basic protein (MBP), tight junction protein zonula occludens 1 (Zo1), TNFα and IL6 were determined by ELISA. The ultrastructural changes of the brain and pancreatic tissues were examined using a transmission electron microscope. Results MBP, Zo1, TNFα and IL6 levels in RES group were lower than those in SAP group at all time points (Plt;0.05). RES and DEX groups had a significantly improved brain pathology compared to SAP group, which had ultrastructural changes such as obvious neuron swelling, capillary hemostasis, thrombosis and cell apoptosis. The parameters did not differ significantly between RES and DEX groups (Pgt;0.05). Conclusion The degradation of Zo1 is involved in the pathophysiology of brain injury in SAP; MBP can be used as a marker of brain injury in SAP rats. Resveratrol can inhibit brain injury associated with SAP. 　　KEY WORDS: severe acute pancreatitis (SAP); resveratrol; brain injury 　　重症急性胰腺炎(severe acute pancreatitis, SAP)发病过程中一旦伴发神经系统并发症，我们就称之为胰性脑病(p