污染土壤中菲降解菌的分离鉴定及其降解能力 Isolation, Identification and Degradation Ability of Phenanthrene-Degrading Bacteria from Contaminated Soils.pdfVIP
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污染土壤中菲降解菌的分离鉴定及其降解能力 Isolation, Identification and Degradation Ability of Phenanthrene-Degrading Bacteria from Contaminated Soils
第22卷第6期 环境科学研究 、u.22.No.6
2009年6月 ResearchofEnvironmentalSciences Jun.,2009
污染土壤中菲降解茵的分离鉴定及其降解能力
杨秀虹,汤婉环,李适宇+,洪 娟
中山大学环境科学与工程学院,广东广州510275
摘要:利用水一硅油双相系统富集培养和平板升华方法,从被多环芳烃(PAHs)污染的表层土壤中筛选分离得到10株能利用
菲为唯一碳源和能源生长的菌株.这10株菌在无机盐培养基中10d内对初始质量浓度为50mg/L的菲的去除率为27.6%一
55.3%,其中一些菌株混合可提高或降低菲的去除率;通过形态观察、生理生化指标测定及分子生物学分析(16SrDNA)等方法
rDNA序列
3个属的相似性达100%,99%和99%.结合分离菌株的形态、生理生化特征和
分别与Gordon/a,Mycobaaeriurn以及Azospirillum
16S
rDNA基因序列的分析结果。初步鉴定菌株PE0402—5为戈登氏菌(Gordon/a
8p.),PEl501—1为固氮螺菌(Azospiriflumsp.).
rDNA
关键词:菲;生物降解;16S
中圈分类号:X172 文献标志码:A 文章编号:1001—6929(2009)06—0675—07
and of
Isolation,Identification
DegradationAbility
BacteriafromContaminatedSoils
YANG Juan
Xiu—hong,7rANGW锄一huan,LI
Shi-yu,HONG
SchoolofEnvironmentalScienceand Yal-∞n 510275。China
Engineerin8,SunUniversity,Guangzhou
bacterial of asasole ofcarbonand for wereisolatedfromsurface
Abstract:Ten source
8h妇capableutilizingphenanthrene energygrowth
soils the withwatersiliconoil
contaminated aromatic selectiveenrichment
bypolycyclic hydrocarbom(PAHs)tIIroughtechnique biphasesystem
andthe of50 in
method.Thesestrainscan with衄initial猢concentration
sublimate-plate de础27.6%-55.3%ofphenanthrene mg,L
mineralmediawithin from80meofthe10 the mteof
10 isolatescaneitherincrease
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