桉树genomic-ssr和est-ssr分子标记的遗传差异性分析.pdf

第34 卷 第3 期 桉树科技 Vol.34 No.3 2017 年9 月 EUCALYPT SCIENCE TECHNOLOGY Sep.2017 桉树Genomic-SSR 和EST-SSR 分子标记的遗传差 异性分析 刘 果，谢耀坚，陈鸿鹏，吴志华 ( 国家林业局桉树研究开发中心, 广东 湛江524022) 摘要：为探讨Genomic-SSR 与EST-SSR 标记在桉树种间的遗传差异，本研究利用两种不同来源的SSR 标记对6 种桉树进行遗传差异性分析。分析结果表明：Genomic-SSR 标记在6 种桉树中具有高的遗传多样性，EST-SSR 标 记在揭示不同基因型间遗传关系方面更具优势。对两种标记进行聚类分析发现，EST-SSR 标记比Genomic-SSR 标 记能更准确地鉴别基因型。两种不同来源 SSR 标记的遗传差异性，为更有效利用 SSR 标记在桉树遗传多样性分 析、种质鉴定等相关研究提供了理论支持。 关键词：桉树；Genomic-SSR；EST-SSR；遗传差异性 中图分类号：S722.3+9 文献标识码：A Genetic Differences Revealed by Genomic-SSRs and EST-SSRs in Eucalyptus Species LIU Guo, XIE Yao-jian, CHEN Hong -peng, WU Zhi-hua (China Eucalypt Research Centre, Zhanjiang 524022,Guangdong,China) Abstract: To explore the genetic differences between genomic-SSR and EST-SSR markers in different species of Eucalyptus, the genetic diversity of 6 species were analyzed using two types of SSR markers in the study reported here. Genomic-SSRs were found to be more polymorphic than EST-SSRs in all 6 Eucalyptus species, with EST-SSRsproving to be more powerful for revealing genetic relationships among different genotypes than genomic-SSRs. Moreover, cluster analyses indicated that EST-SSR markers could identify different genotypes more accurately. The genetic differences revealed in this study for genomic-SSR and EST-SSR markers will provide theoretical support for rational future use of SSR markers in studies of Eucalyptus genetic diversity, germplasm identification and other