磷脂酶c参与para1诱导的烟草悬浮细胞死亡和防卫反应-中国农业科学.pdfVIP

中国农业科学 2010,43(20):4176-4182 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2010.20.007 磷脂酶C参与ParA1诱导的烟草悬浮细胞死亡和防卫反应 魏芳芳，王 蕾，王莲莲，柳金伟，梁元存，王玉军 （山东农业大学植物保护学院，山东泰安 271018 ） 摘要：【目的】研究寄生疫霉Phytophthora parasitica 分泌的蛋白激发子 ParA1 诱导烟草悬浮细胞后，磷 脂酶 C（phospholipase C, PLC）参与 ParA1 诱导烟草过敏细胞死亡（hypersensitive cell death，HCD）和防 卫反应。【方法】采用药理学方法，在烟草悬浮细胞中添加PLC抑制剂，比较烟草悬浮细胞受ParA1诱导后细胞死 亡和多种防卫反应的差别。【结果】PLC 特异性抑制剂 U73122 能完全抑制 ParA1 引起的烟草悬浮细胞死亡，氧迸 发、胞外基质碱化和植保素scopoletin（7-羟基-6-甲氧基香豆素）积累都能显著地被抑制，5种防卫基因 hin1、 hsr203J、PR（pathogenesis-related）-1a、PR-1b 和 PAL 等的转录表达也都受到 U73122 不同程度的抑制。【结 论】ParA1处理烟草悬浮细胞后，PLC参与了ParA1诱导细胞死亡和防卫反应的信号传导过程。 关键词：磷脂酶C; ParA1; 烟草; 细胞死亡; 防卫反应 Involvement of Phospholipase C in Induction of Cell Death and Defense Responses in ParA1-Elicited Tobacco Suspension Cells WEI Fang-fang, WANG Lei, WANG Lian-lian, LIU Jin-wei, LIANG Yuan-cun, WANG Yu-jun (College of Plant Protection, Shandong Agricultural University, Taian 271018, Shandong) Abstract: 【Objective 】 Phospholipase C (PLC) was investigated in response to ParA1, a protein elicitor from Phytophthora parasitica , and could trigger cell death and defense responses in tobacco suspension cells. 【Method 】 Using a pharmacological approach, tobacco suspension cells pretreated with PLC inhibitors (neomycin and U73122) in ParA 1-induced cells were used to compare cell death and a series of defense responses. 【Result 】 Tobacco suspension cells treated with ParA1 resulted in defense responses, such as hypersensitive cell death, an oxidative burst, alkalization of the extracellular medium, expression of 5 defense-related genes and accumulation of scopoletin, defense-related genes hin1, hsr203J, PR-1a, PR-1b and PA