以壳聚糖为佐剂的幽门螺杆菌疫苗的免疫治疗作用.docVIP

以壳聚糖为佐剂的幽门螺杆菌疫苗的免疫治疗作用 谢勇 龚燕锋 周南进 陈江 吕农华 梅承誉 侯晓华 谢 勇，侯晓华，华中科技大学同济医学院附属协和医院华中科技大学同济医学院附属协和医江西省学科带头培养项目,No. K010501 通信作者：候晓华，430022，湖北省武汉市华中科技大学同济医学院附属协和医院 Potential Role of H. pylori vaccine with chitosan on the Immunological Function Yong Xie, Yan-Feng Gong, Nan-Jin Zhou, Jiang Chen, Nong-Hua Lu, Cheng-Yu Mei, Xiao-Hua Hou. Yong Xie, Xioa-Hua Hou ,Union Hospital of Tongji Medical College Huazhong University of Science and Technology, Wuhan 430022, Hubai Province,China Yong Xie, Yan-Feng Gong, Jiang Chen, Nong-Hua Lu, Cheng-Yu Mei, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China Nan-Jin Zhou, Jiangxi Medical Science Institute, Nanchang 330006, Jiangxi Province, China Supported by Natural Science Foundation, No. Program of Jiangxi provincial leader in their chosen field of learning, No. K010501 Correspondence to: Xioa-Hua Hou ,Union Hospital of Tongji Medical College Huazhong University of Science and Technology, Wuhan 430022, Hubai Province,China. houxh@public.wh.hb.cn Telephone:+86-0791-8692507 Abstract AIM: To investigate the Immunological therapeutic role of H. pylori vaccine with chitosan as adjuvant. METHODS: one-grade female Balb/c mice infected H. pylori were established by inoculating with H. pylori, Syndey Strain 1(SS1), and were randomly divided into five groups, including PBS alone as control, H. pylori antigen alone, H. pylori antigen plus chitosan solution, H. pylori antigen plus chitosan particles, and H. pylori antigen plus cholera toxin(CT). The vaccine was orally given once a week for four weeks. At 4 weeks after the last immunization, these mice were killed and gastric mucosa were embedded in paraffin, assayed with Giemsa staining and HE staining. At the same time, H. pylori culture was performed, and an ELISA was used to detect anti-H. pylori IgA in saliva and gastric mucosa and anti-H. pylori IgG in serum. RESULTS: ①In the groups with chitosan as an adjuvant, 58.33% mice could ac