一株费氏丙酸杆菌生长特性及其代谢物抑菌作用分析-食品科学.pdfVIP

※生物工程 食品科学 2015, Vol.36, No.15 163 一株费氏丙酸杆菌生长特性及其代谢物 抑菌作用分析 1,2 2 2 3 1,* 郑丽雪 ，王荆楚 ，时慧佳 ，曹 雪 ，齐 斌 （1.常熟理工学院生物与食品工程学院，江苏 常熟 215500 ；2.苏州市食品生物技术重点实验室， 江苏 常熟 215500 ；3.吉林中粮生化有限公司，玉米深加工国家工程研究中心，吉林 长春 130033 ） 摘  要：对一株费氏丙酸杆菌生长特性及其代谢物抑菌作用进行研究。结果表明：该菌株生长周期约为10 d，第1～3天 为其对数生长期，第4～8天为菌体生长的稳定期，第9～10天为衰亡期。在生长过程中，菌体利用糖的速率很快，还 原糖在发酵1 d内几乎被消耗殆尽，发酵液pH值在发酵的第1天下降到4.6左右，此后一直到第10天，pH值基本稳定在 4.6左右。在同等条件下，该丙酸杆菌代谢物对大肠杆菌的抑制作用优于对金黄色葡萄球菌和酵母菌的抑制作用。对 代谢产物的分析结果表明，发酵产物中抑菌物质除了丙酸之外，还存在其他物质，初步判定为细菌素。 关键词：丙酸杆菌；生长特性；抑菌物质；细菌素 Growth Characteristics and Metabolite Bacteriostatic Action of Propionibacterium freudenreichii 1,2 2 2 3 1,* ZHENG Lixue , WANG Jingchu , SHI Huijia , CAO Xue , QI Bin (1. College of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China; 2. Suzhou Key Laboratory of Food and Biotechnology, Changshu 215500, China; 3. National Engineering Research Center of Corn Deep Processing, Jilin COFCO Bio-chemical Co. Ltd., Changchun 130033, China) Abstract: The growth characteristics and metabolite bacteriostasis of Propionibacterium freudenreichii were studied. The ’ results showed: the strain s growth cycle was approximately 10 days, involving logarithmic phase (1–3 d), stability phase (4–8 d) and decline phase (9–10 d). In the process of growth, sugar was consumed quickly, and almost completely depleted in the first day. The pH after 1 day of fermentation dropped to 4.6 and then remained at 4.6 during the remaining fermen