药分 -.ppt

Detection of dissolved oxygen Significance Detection of dissolved oxygen During the Antibiotic fermentation ,microbial can only obtain oxygen from the liquid matrix for their physiological activities.So we need realtime detection of dissolved oxyge-n.Therefore, diss-olved oxygen is a very important parameter of fermentation parameters【1】. Classification and Principles 2 Measuring parameters 3 Structure and Electrode using 4 Directory OD introduction 1 Optical fluorescence 5 1. OD introduction Dissolved Oxygen（DO）【4】 Its characterization of the concentration of oxygen in aqueous solution parameter Related factors Partial pressure of oxygen Solubility Solution of its own characteristics Determination principle 1、O2 through the membrane permeability infiltration 2、O2 dissolved in the electrolyte ， O2 in the cathodic reduction. 3、REDOX reaction produces the current, the current transmitter into dissolved oxygen values. 1 2 3 Determination method【5】 Instrumental methods：Spectrophotometry, chromatography, electrochemical analysis. Sensor method： Dissolved oxygen electrode Chemical analysis： Iodometric titration Silver anode(+) 4 Ag + 4 Cl- = 4 AgCl + 4 e- Platinum cathode(-) O2 + 2 H2O + 4 e- = 4 OH- Working principle：Polargrafic【2】 Ag+ e- O2 e- Anode Cathode - + Electrolyte Membrane mechanical interface【3】 1 2 3 1 2 Oxygen diffusion through the membrane . 3 4 Oxygen dissolved in the electrolyte Oxygen molecules gradually reduced in the cathode Oxidation reaction current 3. What is measured Oxygen concentration Sensor current relationship with the following parameters (stable area) I: Sensor current[nA] n: The number of electron mobility(n = 4) F: Faraday constant(F = 96.485 C/mol) A: Cathode surface area [cm2] D: Oxygen diffusion coefficient [cm2/s] S: Membrane solubility of oxygen[mol/(cm3*bar)] pO2: Polarization voltage of oxygen[bar] d: Film thickness[cm] DO electrode dissolved oxygen concentration is not measured, b