Identification of Altered MicroRNA Expression Patterns in Synovial Sarcoma英文文献.pdfVIP

GENES, CHROMOSOMES CANCER 50:137–145 (2011) RESEARCH ARTICLES Identification of Altered MicroRNA Expression Patterns in Synovial Sarcoma 1 1 1 2 3 4 Masanori Hisaoka, * Atsuji Matsuyama, Yuichi Nagao, Lan Luan, Toshihiko Kuroda, Hideo Akiyama, Satoshi Kondo,3 and Hiroshi Hashimoto 1 1 Department of Pathology and Oncology, School of Medicine,Universityof Occupational and Environmental Health, Kitakyushu,Japan 2 Department of Pathology,Fengtian Hospital, Shenyang Medical Collage,Liaoning Province,China 3 New Products Development Division,Toray Industries,Inc.,Kamakura,Japan 4The New Frontiers Research Laboratories,Toray Industries,Inc.,Kamakura,Japan MicroRNAs (miRNAs) are noncoding small RNAs that function as an endogenous regulator of gene expression. Their dys- regulation has been implicated in the development of several cancers. However, the status of miRNA in soft tissue sarco- mas has not yet been thoroughly investigated. This study examined the global miRNA expression in synovial sarcoma and compared the results to those in another translocation-associated sarcoma, the Ewing family of tumors, and in normal skeletal muscle. The 3D-Gene miRNA microarray platform (Toray, Kamakura, Japan) and unsupervised hierarchical cluster- ing revealed a distinct expression pattern of miRNAs in synovial sarcoma from Ewing tumors and skeletal muscle. Thirty- five of the more than 700 miRNAs analyzed were differentially expressed in synovial sarcomas in comparison to other tis- sue types. There were 21 significantly up-regulated miRNAs, including some miRNAs, such as let-7e, miR-99b, and miR- 125a-3p, cluster