原创力文档Bacterial community characteristics under long term antibiotic selection pressures英文文献.pdfVIP
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w a t e r r e s e a r c h 4 5 ( 2 0 1 1 ) 6 0 6 3 e6 0 7 3
Available online at
journal homepage: /locate/watres
Bacterial community characteristics under long-term
antibiotic selection pressures
Dong Li, Rong Qi, Min Yang*, Yu Zhang, Tao Yu
State Key Lab of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing
100085, China
a r t i c l e i n f o a b s t r a c t
Article history: To investigate bacterial community characteristics under long-term antibiotic selection
Received 25 February 2011 pressures, water samples from the upstream and the downstream sections of two rivers
Received in revised form individually receiving the treated penicillin G and oxytetracycline production wastewater,
30 August 2011 as well as the anaerobic and the aerobic effluent of the penicillin G production wastewater
Accepted 1 September 2011 treatment plant, were taken and analyzed. Antibiotic resistance ratios of bacterial
Available online 10 September 2011 communities in water samples were estimated by culture-based analysis. The majority of
bacterial colonies (approximately 55%e70%) in both downstream rivers and the aerobic
Keywords: effluent showed resistance to 80 mg/ml of antibiotics tested, while the resistance ratios
Antibiotic resistance were less than 10% and 5% respectively for both upstream rivers. Six 16S rRNA gene clone
Bacterial community libraries were constr
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