Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation英文文献.pdfVIP

Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation a,b,1 c,1 a,b a,b d e Suk-Jin Ha , Jonathan M. Galazka , Soo Rin Kim , Jin-Ho Choi , Xiaomin Yang , Jin-Ho Seo , N. Louise Glassf, Jamie H. D. Catec,g,2, and Yong-Su Jina,b,2 a Department of Food Science and Human Nutrition, University of Illinois, Urbana, IL 61801; bInstitute for Genomic Biology, University of Illinois, Urbana, IL 61801; c Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720; d BP Biofuels Business Unit, Berkeley, CA 97420; e Department of Agricultural Biotechnology, Seoul National University, Seoul 152-742, Korea; f Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720; and g Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720 Edited by Lonnie ONeal Ingram, University of Florida, Gainesville, FL, and approved December 1, 2010 (received for review July 22, 2010) The use of plant biomass for biofuel production will require effi- yield of xylose fermentation in S. cerevisiae. Despite these endea- cient utilization of the sugars in lignocellulose, primarily glucose vors, reported ethanol yields and productivities from xylose and xylose. However, strains of Saccharomyces cerevisiae presently fermentation by engineered strains were much inferior to those used in bioethanol production ferment glucose but not xylose. of xylose fermentation by P. stipitis , as well as those of glucose Yeasts engineered to ferment xylose do so slowly, and cannot fermentation by S. cerevisiae (10, 11). Moreover, both natural utilize xylose until glucose is completely consumed. To overcome