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CHAPTER 1
Heterologous Protein Secretion
by Bacillus Species: From the
Cradle to the Grave
Susanne Pohl and Colin R. Harwood1
Contents I. Introduction 2
II. Expression and Secretion Vector Systems 3
III. Bacillus Secretion Pathways 5
IV. Substrate Recognition 5
V. Intracellular Chaperoning and Piloting to the
Sec Translocase 7
VI. SecA and the Sec Translocase 10
VII. Cell Wall Structure and Implications for Secretion 11
VIII. Posttranslocation Folding 12
A. Propeptides 13
B. Divalent cations as folding catalysts 14
C. Peptidyl prolyl cis/trans isomerases 15
D. Disulfide isomerases 15
IX. Membrane-Bound and Extracellular Proteases 16
X. Concluding Remarks 18
Acknowledgments 20
References 20
Abstract The Gram-positive bacterium Bacillus subtilis
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