原创力文档Effect of pyrene on denitrification activity and abundance and composition of denitrifying community in an agricultural soil英文文献.pdfVIP
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Environmental Pollution 159 (2011) 1886e1895
Contents lists available at ScienceDirect
Environmental Pollution
journal homepage: /locate/envpol
Effect of pyrene on denitrification activity and abundance and composition
of denitrifying community in an agricultural soil
Guang-Xia Guo a, Huan Deng a, Min Qiao a, Yu-Jing Mu a, Yong-Guan Zhu a, b, *
a State Key Lab of Regional and Urban Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
b Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China
a r t i c l e i n f o a b s t r a c t
Article history: Toxicity of pyrene on the denitrifiers was studied by spiking an agricultural soil with pyrene to a series of
Received 25 October 2010 concentrations (0e500 mg kg1) followed by doseeresponse and dynamic incubation experiments.
Received in revised form Results showed a positive correlation between potential denitrification activity and copy numbers of
18 March 2011
denitrifying functional genes (nirK, nirS and nosZ), and were both negatively correlated with pyrene
Accepted 19 March 2011
concentrations. Based on the comparison of EC50 values, denitrifiers harboring nirK, nirS or nosZ gene
were more sensitive than denitrification activity, and denitrifiers harboring nirS gene were more
Keywords:
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