A drastic reduction in F1 transcript levels does not affect C4 specific gene expression in maize英文文献.pdfVIP

ARTICLE IN PRESS Journal of Plant Physiology 164 (2007) 1665—1674 www.elsevier.de/jplph A drastic reduction in DOF1 transcript levels does not affect C4-specific gene expression in maize$ Markus Cavalar, Yvonne Phlippen, Fritz Kreuzaler, Christoph Peterha¨nsel RWTH Aachen, Institute for Biology I, Worringer Weg 1, 52056 Aachen, Germany Received 26 April 2006; accepted 12 September 2006 KEYWORDS Summary C4 -metabolism; The transcription factor DOF1 has been suggested to regulate photosynthetic gene DOF1; expression in maize. By screening a RescueMu transposon-tagged mutant library, we Maize; identified a maize mutant with a transposon integration in the Dof1 gene 16 bp PEPC; upstream of the transcription initiation site (TIS). Sequencing of the Dof1 promoter RescueMu region revealed an unusual promoter structure missing any typical elements. Homozygous (ho) mutant lines were generated by selfing and subsequent PCR and DNA gel blot analyses. The transposon integration reduced Dof1 transcript levels to less than 20% compared to the wild-type and overlapping RT-PCR systems revealed that these transcripts were not initiated from the native transcription start site. Dof1 transcripts transiently accumulate in wild-type plants after illumination of darkened seedlings, but this ac