耐碳青霉烯类鲍曼不动杆菌噬菌体AB3的基因组生物信息分析研究.DOCVIP

鲍曼不动杆菌噬菌体AB3的全基因组测序及生物信息学分析 张 劼，刘 茜,甘 丹 (400014 重庆，重庆市第三人民医院老年科) [摘要] 目的 对本研究小组分离的鲍曼不动杆菌EditSeq、tRNAscan-SE、TRF、FindTerm、ORF finder、BPROM、GeneMarkTM、Clustalx、phylip等软件对所获噬菌体AB3基因组的一般特性、编码基因的功能预测、RNA聚合酶基因系统的进化进行分析。结果 噬菌体AB3基因组为全长31 185 bp的双链DNA，G+C mol%为39.18%，包含28个预测基因，1个转录终止子和4个可能的启动子序列。结论 基因分析和RNA聚合酶基因进化分析显示噬菌体AB3与噬菌体AB1类似，均属于phiKMV-like病毒属。 [关键词] 鲍曼不动杆菌；噬菌体；基因组；生物信息学 [中图分法分类号] Q939 [文献标志码] A Sequencing of Acinetobacter baumannii bacteriophage genome AB3 and analysis of its bioinformatics ZHANG Jie, LIU Xi , GAN Dan (Department of Geriatrics Medicine, The Third People’s Hospital of Chongqing, Chongqing 400014, China) [Abstract] Objective To sequence Acinetobacter baumannii bacteriophage AB3 separated by the lab and analyze its bioinformatics, so as to lay a foundation of the genome remodeling and bacteriophage therapy. Methods Shot-gun library and contig package strategy were carried out for sequencing bacteriophage AB3 genome. Such software as EditSeq, tRNAscan-SE, TRF, FindTerm, ORF finder, BPROM and GeneMarkTM were applied to predict both general characteristics of the bacteriophage AB3 genome and coding gene function. In addition, the evolution of RNA polymerase gene system was analyzed with software of Clustalx and phylip. Results The genome of bacteriophage AB3 was a double-strand DNA with a full length of 31,185bp, in which G+C mol% was 39.18% and 28 predicted genes, 1 transcription terminator, and 4 possible promoter sequences were included. Conclusions Analysis of gene and RNA polymerase gene evolution indicate that bacteriophage AB3 is similar to bacteriophage AB1 and both belong to phiKMV-like virus. [Key Words] Acinetobacter baumannii; Bacteriphage; Genome; Bioinformatic Corresponding author: Liu Xi, E-mail：Lixj960054@163.com [基金项目] 重庆市卫生局科研资助(2009-2-081,2011-2-334, 2012-2-218) [通信作者] 刘 茜, E-mail：Lixj960054@163.com 鲍曼不动杆菌对常用的抗菌药物具有广泛的耐药性，患者往往死于无药可治。根据2010年中国CHINET细菌耐药性监测网数据显示，鲍曼不动杆菌占临床分离革兰阴性菌的16.11