Introduction to Genetic Analysis 9e：遗传分析技巧介绍.pptVIP

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Introduction to Genetic Analysis 9e：遗传分析技巧介绍.ppt

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Introduction to Genetic Analysis 9e：遗传分析技巧介绍

CHAPTER 20 Gene Isolation and Manipulation CHAPTER OUTLINE 20.1 Generating recombinant DNA molecules 20.2 DNA amplification in vitro: the polymerase chain reaction 20.3 Determining the base sequence of a DNA segment 20.4 Forward genetic analysis by using positional cloning 20.5 Detecting human disease alleles: molecular genetic diagnostics 20.6 Genetic engineering * Copyright 2008 ? W H Freeman and Company Injection of foreign DNA into an animal cell Chapter 20 Opener The progenitor of domesticated corn Figure 20-1 Formation of a recombinant DNA molecule Figure 20-2 Inserting a gene into a recombinant DNA plasmid Figure 20-3 How amplification works Figure 20-4 A plasmid vector, pUC18 Figure 20-5 Cloning in phage ? Figure 20-6 Structure of a BAC vector Figure 20-7 Modes of delivering recombinant DNA into bacterial cells Figure 20-8 Finding the clone of interest by using DNA or RNA probes Figure 20-9 Finding the clone of interest by using antibody Figure 20-10 Finding the clone of interest by using antibody Figure 20-10 part 1 Finding the clone of interest by using antibody Figure 20-10 part 2 Gel electrophoresis Figure 20-11 Finding specific nucleic acids by using gel electrophoresis and blotting Figure 20-12 Finding specific nucleic acids by using gel electrophoresis and blotting Figure 20-12 part 1 Finding specific nucleic acids by using gel electrophoresis and blotting Figure 20-12 part 2 Chromosome walking Figure 20-13 *