马铃薯离体块繁的实践与应用演示幻灯片.pptVIP

Medium ? Culture environment conditions? Discussions MS+0.2BA + 10mg D-泛酸钙 +3%蔗糖； MS + 3% sugar + 0.01% C; 20-25℃，3000-4000LX，14-16hr光照； 每3周继代一次，单芽茎段约1厘米； 原则试管苗用2年—3年。 马铃薯继代培养基： After culturing for 3-4 weeks, elongated shoots are transferred to multiplication medium Stage 2 4 weeks 分割移植 ×3 ×6 Principle of multiplicationNodal proliferation， Axillary bud formationOne nodal cutting one plant，high genetics，simple operation, higher survival ex-vitro，but lower multiplication rate at beginning. ×6 ×3 6 shoots 6 shoots Nodal cuttings CUT CUT Axillary bud formation Restrain top-bud growth, enhance formations of axilary buds High genetics，simple operation Axillary branching Next explant CUT Principle of multiplication Young shoots or bud grown on a appropriate culture medium become cluster shoots. Higher multiplication rate with higher mutation when high phytohormone used. Shoot proliferation Elongation and rooting 3 stage Shoot elongation Rooting Elongation medium Rooting medium Stage 3 A. Shoot elongationB. Root induction development Medium ? Culture environment conditions? Discussions MS+NAA0.2+D-泛酸钙10mg +3%蔗糖； MS (surge-free medium); 20-25℃，3000-4000LX，14-16hr光照； 每3周继代一次，单芽茎段约1厘米； 原则试管苗用2年—3年。 马铃薯生根培养： Stage 3 Stage 4 へ 4 weeks 発根する Rooting mediumFull strength MS mediumIBA 0.5 mg L-1 vermiculite 100ml/vessel 伸長したシュート 　　　　　At this stage, rooted plantlets are transferred to a cell tray and grown under 60% shaded and high RH conditions for 7 to 10 days to acclimatize Acclimatization are usually attained in 2 to 3 weeks Acclimatization Acclimatization 4 stage From in vitro environment to ex vitro environment From photomixotrophy to photoautotrophy Carbon source: sugar in the media Aseptic environment Low light intensity High relative humidity Carbon source: CO2 in the air Nonaseptic environment high light intensity Low relative humidity In vitro environment Ex vivo environment Acclimatization 炼苗室温度：白天23~27℃，夜间不低于14℃。 炼苗具体方法：移植前7d左右，将长有3~5