survivin反义寡核苷酸对乳腺癌mcf-7细胞株mmp-2及-江苏大学.docVIP

生存素反义寡核苷酸对乳腺癌MCF-7细胞株MMP-2及MMP-9表达的影响 许1，肖，霞2，燕2，蒋2，于2 （泰州市人民医院普外科，2.江苏大学附属人民医院病理科，江苏 212002） 【摘要】目的：研究生存素（survivin）反义寡核苷酸（antisense oligodeoxynucleotide，ASODN）对人乳腺癌细胞株MCF-7基质金属蛋白酶-2（matrix metalloproteinase-2，MMP-2）及基质金属蛋白酶-9（matrix metalloproteinase-9，MMP-9）表达的影响，以进一步阐明生存素介导乳腺癌浸润及转移的机制。方法：经脂质体介导将生存素 ASODN转染人乳腺癌细胞株MCF-7，用荧光显微镜检测转染效率，用逆转录-聚合酶链反应（RT-PCR）、蛋白质印迹法检测转染细胞生存素、MMP-2及MMP-9表达改变。结果：荧光显微镜检测结果显示生存素 ASODN可成功转染MCF-7细胞。与对照组相比，转染组细胞生存素、MMP-2、MMP-9 mRNA和蛋白的表达均显著降低。结论：生存素 ASODN转染可有效抑制MCF-7细胞生存素基因的表达，并降低其MMP-2、MMP-9表达，可能对乳腺癌具有基因治疗的效果。 【关键词】生存素；反义寡核苷酸；基质金属蛋白酶-2；基质金属蛋白酶-9；乳腺癌 Effect of survivin ASODN transfection on the expression of MMP-2 and MMP-9 in human breast cancer cell XU Wen-min1，XIAO ， Xia2,LI Yan2，JIANG -tao2，YU 2 （1.Department of General Surgery, Taizhou People’s Hospital, Taizhou Jiangsu 225000 2.Department of Pathology, Affiliated People’s Hospital of Jinagsu University, Zhenjiang Jiangsu 221002，China） [Abstract] Objective：To investigate the effect of survivin antisense oligodeoxynucleotide（ASODN） transfection on the expression of matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase -9(MMP-9) in human breast cancer line MCF-7 and to provide experimental foundation for survivin ASODN gene therapy for breast cancer. Methods: Survivin ASODN was transfected into MCF-7 cells through lipofectin. The rate of transfection was detected by fluorescence microscopy. The expressions of survivin, MMP-2 and MMP-9 in transfected cells were detected by RT-PCR and Western blot. Results: Survivin ASODN was successfully transfected into MCF-7 cells. After the transfection, RT-PCR and Western blot analysis revealed that the expressions of survivin, MMP-2 and MMP-9 mRNA and their proteins of antisense group was decreased significantly, compared with the control group . Conclusion: The transfection of survivin ASODN could effectively down-regulate the expressions of survivin, MMP-2 and MMP-9 in MCF-7 cell. Survivin ASODN may provide potential gene therapy for breast