阿莫西林与甲基紫的共振瑞利散射及应用-中国现代应用药学.PDFVIP

·药物分析与检验 · 阿莫西林与甲基紫的共振瑞利散射及应用 江虹，庞向东，周国静(长江师范学院化学化工学院，重庆 408100) 摘要：目的 建立测定痕量阿莫西林的共振瑞利散射法。方法 以甲基紫作探针的共振瑞利散射法测定阿莫西林。结果 在 pH 9.90 的 Tris-HCl 溶液中，阿莫西林和甲基紫相互作用后，共振瑞利散射显著增强，在 376 nm 处的△IRRS 最强。阿 莫西林的浓度在 8 6 1 1 2.0 ×10 ~1.5 ×10 mol·L 内与 △I 成正比，检出限(3S /S) 为 0.042 µg·mL 。结论 该法简便、快 RRS b 速、灵敏，可用于痕量阿莫西林的测定。 关键词：共振瑞利散射；阿莫西林；甲基紫 中图分类号：R978.11 ；R917.102 文献标志码：B 文章编号：1007-7693(2010)11-1021-04 Resonance Rayleigh Scattering Spectra of Interaction of Amoxicillin with Methyl Violet and Their Analytical Applications JIANG Hong, PANG Xiangdong, ZHOU Guojing(College of Chemistry and Chemical Engineering, Yangtze Normal University, Chongqing 408100, China) ABSTRACT: OBJECTIVE To develop a new sensitive Resonance Rayleigh scattering(RRS) method for the determination of trace amoxicillin. METHODS RRS spectra method was used for the determination of amoxicillin with methyl violet as probe. RESULTS In pH 9.90 Tris-HCl buffer solution, the RRS intensity of methyl violet was greatly enhanced when amoxicillin was added. And the strongest ΔIRRS was located at 376 nm. The intensity of RRS was in proportion to the concentration of 8 6 1 1 amoxicillin in the range of 2.0×10 1.5×10 mol·L with a detection limit(3S /S) of 0.042 µg·mL . CONCLUSION The b method showed advantage of convenience, rapidity, high sensitivity and was applied in the determination of trace amoxicillin with suitable results. KEY WORDS: Resonance Rayleigh scattering; amoxicillin; methyl violet 阿莫西林(AMO)是一种广谱、不耐青霉素酶 了测定AMO 的新方法。该法简便、快速、灵敏， 的半合成青霉素，其作用较氨苄青霉素强，