棉花品种遗传纯度的SSR分子标记鉴定技术研究精品.pdfVIP

花 学 报 Cotton Science 20 13 25 渊5 冤院382~387 花品种遗传纯度的SSR 分子标记鉴定技术研究 1 1 1 2 1* 国栋 王芙蓉 宫永超 马和欢 张 军 (1. 农业部黄淮海棉花遗传改良与栽培生理重点实验室 山东棉花研究中心 济南250100 曰2. 山东师范大学生命科学学 院 济南250014) 摘要院为建立适合于SSR 标记的棉花品种纯度鉴定方法 利用78 个SSR 标记对12 个棉花常规品种进行标记 基因型分析遥 通过比较不同品种不同单株标记基因型 发现棉花品种的非纯SSR 位点存在3 种主要类型遥 通 过综合分析非纯SSR 位点率和异型单株率对品种遗传纯度的影响 建立了利用SSR 标记在分子水平上鉴定 棉花品种纯度的方法遥 利用该方法鉴定的12 个棉花品种中 3 个品种渊C5 尧C9 和C 11冤的遗传纯度在98% 以 上 非纯SSR 位点和异型株均较高的2 个品种渊C3 和C6冤遗传纯度分别为67.3 1%和31.79% 该方法弥补了以 往分子纯度计算方法中只考虑单株混杂尧不考虑SSR 位点混杂的缺陷 可比较客观地反映品种的遗传纯度状况遥 关键词院棉花品种曰SSR 曰非纯位点曰遗传纯度 中图分类号院S562.035 文献标志码院A 文章编号院1002-7807 渊20 13冤05-0382-06 1 1 1 2 1* LIU Guo-dong , WANG Fu-rong , GONG Yong-chao , MA He-huan , ZHANG Jun (1. 250100, ; 2. 250014 ) To establish a method for genetic purity identification of cotton ( L.) varieties by SSR markers, marker genotypes of 12 conventional cotton genotype varieties were surveyed using 78 pairs of core SSR primers. By comparing SSR loci from different individuals of all tested cotton varieties, the non-homozygous SSR alleles of cotton variety were divided into three typical scenarios at the molecular level. Consequently, a method for the genetic purity identification of cotton varieties using SSR markers was proposed based upon analysis of the comprehensive influences of both non-homozygous SSR loci and the rate of heterotype individuals in the cotton varieties. For the genetic purity of 12 cotton varieties tested by this method, three varieties (C5, C9 and C 11) were above 98% , while two varieties (C3 and C6) were 67.31% and 3 1.79% , respectively. The method proposed takes into account b