干细胞研究进展KeepUpdated-ThermoFisherScientific.PDFVIP

干细胞研究进展（Keep Updated） Laboratory Solution China Thermo Fisher Scientific Cell Rep：人胚胎干细胞可被诱导成为精子前体细胞 Human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have been shown to differentiate into primordial germ cells (PGCs) but not into spermatogonia, haploid spermatocytes, or spermatids. Here, we show that hESCs and hiPSCs differentiate directly into advanced male germ cell lineages, including postmeiotic, spermatid-like cells, in vitro without genetic manipulation. Furthermore, our procedure mirrors spermatogenesis in vivo by differentiating PSCs into UTF1-, PLZF-, and CDH1-positive spermatogonia-like cells; HIWI- and HILI positive spermatocyte-like cells; and haploid cells expressing acrosin, transition protein 1, and protamine 1 (proteins that are uniquely found in spermatids and/or sperm). These spermatids show uniparental genomic imprints similar to those of human sperm on two loci: H19 and IGF2. These results demonstrate that male PSCs have the ability to differentiate directly into advanced germ cell lineages and may represent a novel strategy for studying spermatogenesis in vitro. Reference: Easley et al., Direct Differentiation of Human Pluripotent Stem Cells into Haploid Spermatogenic Cells, Cell Reports (2012), /10.1016/j.celrep.2012.07.015 Cell Rep：人胚胎干细胞可被诱导成为精子前体细胞 2012年8月29 日迅匹兹堡大学医学院研究人员证实：人类胚胎干 细胞和人诱导性多能干细胞（iPS细胞）可以被诱导成为精子前体 细胞，这表明它未来可能用于恢复不育男性的生育能力，他们的 研究结果将发表在Cell Reports杂志上。 研究报告的主要作者、匹兹堡大学医学院妇科和生殖科学 Charles Easley博士解释说：不育可能是某些癌症治疗所带来的副 作用，因为药物是通过破坏迅速分裂的细胞来发挥作用，这些迅 速分裂的细胞就包括精子的前体细胞。 有越来越多的研究证据证实成年体细胞如皮肤中的细胞可以 诱发演变返回到原始状态，然后重新定向到成为不同类型的细胞。 为了探究是否能够获得精子细胞，Easley博士和他的同事们从市售 的皮肤样本中获取培养实验室级的人iPS细胞，同时从已建立的细 胞系中获取培养人胚胎干细胞，在通常情况下，人胚胎干细胞主 要用于维持精原干细胞。 他们发现，两种干细胞能够产生一些关键性细胞包括精原干 细胞、包含了细胞减数分裂前完整染色体的精母细胞，染色