Lindane embryotoxicity and differential alteration of cysteine…林丹胚胎毒性和半胱氨酸差异性改变.pdfVIP

Reproductive Toxicology, Vol. 8, No. 4, pp. 351-362, 1994 Pergamon Copyright © 1994Elsevier Science Ltd Printed in the USA. All fights reserved 0890-6238/94 $6.00 + .00 0890-6238(94)E0021-M L I N D A N E E M B R Y O T O X I C I T Y A N D D I F F E R E N T I A L A L T E R A T I O N O F C Y S T E I N E A N D G L U T A T H I O N E L E V E L S I N R A T E M B R Y O S A N D V I S C E R A L Y O L K S A C S TAMARA L. MCNUTT and CRAIG HARRIS Toxicology Program, Department of Environmental and Industrial Health, The University of Michigan, Ann Arbor, MI Abstract m The lindane embryotoxicity and associated changes in cysteine (CYS) and glutathione (GSH) status have been investigated in the early organogenesis-stage rat conceptus utilizing whole embryo culture techniques. Direct exposure of gestational day 10 (GD 10) conceptuses to lindane (50, 100, 200, 300, and 400 pM) in the culture medium resulted in a dose- and time-dependent increase in mortality (88% at 400 pM), frequency, and severity of malformations and in decreased growth parameters. Protein and DNA contents of embryo and