Localization of Superoxide Dismutases and Hydrogen Peroxide in…超氧化物歧化酶和过氧化氢在中的定位.pdfVIP

MPMI Vol. 17, No. 12, 2004, pp. 1294–1305. Publication no. M-2004-0907-02R. © 2004 The American Phytopathological Society Localization of Superoxide Dismutases and Hydrogen Peroxide in Legume Root Nodules 1 2 1 3 3 Maria C. Rubio, Euan K. James, Maria R. Clemente, Bruna Bucciarelli, Maria Fedorova, Carroll P. Vance,3 and Manuel Becana1 1Departamento de Nutrición Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas, Apdo 202, 50080 Zaragoza, Spain; 2Centre for High Resolution Imaging and Processing, MSI/WTB Complex, School of Life Sciences, University of Dundee, Dundee DD1 5EH, U.K.; 3 Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, 1991 Upper Buford Circle, St Paul, Minnesota 55108, U.S.A. Submitted 9 April 2004. Accepted 2 July 2004. Superoxide dismutases (SODs) catalyze the dismutation of tial toxic effects of superoxide radicals, nodules contain high superoxide radicals to O and H O and thus represent a levels of superoxide dismutases (SODs). These enzymes cata- 2 2 2 primary line of antioxidant defense in all aerobic organ- lyze the dismutation of the superoxide radicals and thus repre- isms. H2O2 is a signal molecule involved in the plant’s re- sent a primary line of protection against oxidative damage. The sponse to pathogen attack and other stress conditions as information on the distribution of SODs in nodules is, however, well as in nodulation. In this work, we have tested the hy- scant and based mainly on assays of SOD activity in purified or- pothesis that SODs are a source of