Reexamination of the pattern of geographical disjunction of…重新审视的地理分离格局.pdfVIP

Botanical Studies (2010) 51: 511-520. ECOLOGY Reexamination of the pattern of geographical disjunction of Chamaecyparis (Cupressaceae) in North America and East Asia 1,2 3 1, Pei-Chun LIAO , Tsan-Piao LIN , and Shih-Ying HWANG * 1Department of Life Science, National Taiwan Normal University, 88 Ting-Chow Road, Section 4, Taipei 11677, Taiwan 2 Department of Life Science, National Pingtung University of Science and Technology, 1 Shuefu Road, Neipu, Pingtung 912, Taiwan 3Institute of Plant Biology, National Taiwan University, 1 Roosevelt Road, Section 4, Taipei 10617, Taiwan (Received November 24, 2008; Accepted March 17, 2010) ABSTRACT. The genus Chamaecyparis (Cupressaceae) is comprised of six taxa native to East Asia and North America. Two independent lineages from eastern North America to East Asia and from western North America to East Asia have been identified from phylogenetic analyses based on variation in plastid DNA (ptDNA). However, the trees inferred from a nuclear ribosomal internal transcribed spacer (nrITS), the NEEDLY intron 2, and combined data from genetic and morphological characters of other studies are incongruent with the tree inferred from ptDNA. In this study, we sequenced the plastid matK gene as well as other sequences acquired from GenBank including the matK, rbcL, and nrITS sequences to reconstruct the phylogenetic trees of Chamaecyparis. The phylogenetic topology inferred from matK was congruent with the previous inference obtained using other ptDNA markers while the rbcL and ITS trees were congruent with previously inferred trees using the combined data and ITS sequences, respectively. Relatively less- (rbcL) and