不同培养基及培养条件对单增李斯特菌ERIC-PCR-现代食品科技.PDFVIP

现代食品科技 Modern Food Science and Technology 2017, Vol.33, No.5 不同培养基及培养条件对单增李斯特菌ERIC-PCR 分子分型的影响 赵晨晖，丁承超，刘箐 （上海理工大学医疗器械与食品学院，上海 200093） 摘要：本研究以实验室保存的 26 株李斯特氏菌为实验对象，通过 ERIC-PCR 和凝胶电泳技术对其展开基因分型，并根据分型结 果选取不同亚型菌株，进而考虑改变细菌培养条件来探究不同培养基对李斯特菌 ERIC-PCR 分子分型结果的影响。实验结果初步表明， 实验室26 株李斯特菌至少可分成 11 个主要基因类群，其中 Ⅰ型菌株最多占有 8 株，而Ⅲ、Ⅶ、Ⅷ、Ⅸ、Ⅹ以及Ⅺ型均最少，各占 1 株；同时发现，培养基及培养条件的改变会对 LM87、LM109 （野生型单增李斯特菌）分型结果产生影响，且这些菌株分型均最少。 由此推断，不同的营养机制和培养条件可能会对李斯特菌的基因组稳定性或基因表达带来潜在影响，因此无论是在对LM 致病机理的 基础研究过程中还是对其进行分子分型鉴定，都必须考虑其寄主来源以及培养条件。 关键词：培养基；单核细胞增生性李斯特菌；ERIC-PCR；分子分型 文章篇号：1673-9078(2017)5-115-120 DOI: 10.13982/j.mfst.1673-9078.2017.5.019 Influence of Different Medium Conditions on Molecular Typing of Listeria monocytogenes by Enterobacterial Repetitive Intergenic Consensus PCR ZHAO Chen-hui, DING Cheng-chao, LIU Qing (School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China) Abstract: Twenty-six strains of Listeria spp. stored in the laboratory were used as the experimental object here, and the enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) and gel electrophoresis techniques were applied to genotyping. Furthermore, according to the typing results, strains of different subtypes were selected, and the effects of different medium conditions on the results of ERIC-PCR molecular typing of Listeria spp. were explored by changing the culture conditions. The preliminary test results showed that 26 strains of Listeria spp. in the laboratory could be su