A comparative analysis of genetic diversity in medicinal Chrysanthemumn推荐.pdfVIP

Biochemical Systematics and Ecology 38 (2010) 1160–1169 Contents lists available at ScienceDirect Biochemical Systematics and Ecology journal homepage: www.elsevier .com/locate/biochemsyseco A comparative analysis of genetic diversity in medicinal Chrysanthemum morifolium based on morphology, ISSR and SRAP markers Qing-Song Shao a, b, Qiao-Sheng Guo a, *, Yan-Ming Deng c, Hai-Peng Guo b a Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, PR China b Zhejiang A F University, Hangzhou 311300, PR China cJiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China a r t i c l e i n f o a b s t r a c t Article history: The diversity and genetic relationship among 29 populations of Chrysanthemum mor- Received 19 May 2010 ifolium, one of Chrysanthemum indicum and one of Chrysanthemum nankingense from China Accepted 9 November 2010 were analyzed using morphological traits and molecular markers. Twenty morphological Available online 4 December 2010 traits were scored as well as 182 ISSR marker-fragments, as amplified by 22 primers [the percentage of polymorphic bands (PPB): 81.87%], and 243 SRAP marker-fragments as Keywords: generated by 26 primer pairs (PPB: 75.72%). Mantel’s test indicated significant correlation Chrysanthemum morifolium (r ¼ 0.624) of morphological trait and SRAP. By contrast, the morphological trait showed Genetic diversity Morphological traits