UPLC法测定新生脉散液中紫丁香苷的含量Δ.pdf

UPLC 法测定新生脉散液中紫丁香苷的含量Δ 1＊ 2# 2 2 刘建海 ，崔维利 ，陈 涛 ，孟 萌 （1.天津中医药大学研究生院，天津 300193；2.天津中医药大学第一附属 医院，天津 300193） 中图分类号 R927.2 文献标志码 A 文章编号 1001-0408（2015）09-1236-03 DOI 10.6039/j.issn.1001-0408.2015.09.29 摘 要 目的：建立测定新生脉散液中紫丁香苷含量的方法。方法：采用超高效液相色谱（UPLC ）法。色谱柱为ACQUITY UPLC BEN C18，流动相为乙腈-0.4％磷酸水溶液（5∶95，V/ V），流速为0.2ml/min ，检测波长为265nm ，柱温为30℃，进样量为1μl。 结果：紫丁香苷的进样量在0.0197～0.1970μg 范围内与峰面积呈良好的线性关系（r ＝0.9999）；精密度、稳定性、重复性试验的 RSD 均＜2％；平均加样回收率为99.01％，RSD ＝1.27％（n ＝6）。结论：该方法结果准确、重复性好，可作为新生脉散液中紫丁香 苷的含量测定方法。 关键词 新生脉散液；超高效液相色谱法；紫丁香苷；含量测定 Content Determination of Syringin in New Shengmaisan Liquid by UPLC 1 2 2 2 LIU Jian-hai ，CUI Wei-li ，CHEN Tao ，MENG Meng （1.Graduate School ，Tianjin University of TCM ，Tianjin 300193，China ；2.The First Hospital Affiliated to Tianjin University of TCM ，Tianjin 300193，China ） ABSTRACT OBJECTIVE ：To establish a method for content determination of syringin in New Shengmaisan liquid. METHODS ： UPLC method was adopted. The determination was performed on ACQUITY UPLC BEN C18 column with mobile phase consisted of acetonitrile-0.4％phosphoric acid （5∶95，V/ V）at the flow rate of 0.2ml/min. The detection wavelength was 265nm ，the column temperature was 30 ℃and the sampling volume was 1μl. RESULTS ：There was a good linear relationship between the amount of syringin and peak area in the range of 0.019 7-0.1970 μg （r ＝0.999 9）；the RSDs of precision ，stability and repeatability tests was less than 2％；the average recovery was 99.01％（RSD ＝1.27％，n ＝6）. CONCLUSIONS ：The method is accurate and reproducible. It can be used for content determination of syringin in New Shengmaisan liquid. KEYWORDS New Shengmaisan Liquid ；UPLC ；Syringin ；Content determination 新生脉散液是天津中医药大学第一附属医院自制制剂， 型电子分析天平（瑞士Mettler-Toledo 公司）；KQ-250E 型台式 由刺五加、黄芪、丹参、党参等多味中药材提取加工制成，具有 超声波清洗机（昆山超声仪器有限公司）；DZKW-S