BT型三系杂交粳稻不同组合间育性差异原因初探教案.docVIP

BT型三系杂交粳稻不同组合间育性差异原因初探 张宏根＃　钱凯＃　孙一标　裴艳　封智蔷　刘晓宇　汤述翥?　梁国华　顾铭洪 (扬州大学江苏省作物遗传生理重点实验室/教育部植物功能基因组学重点实验室,江苏扬州２２５００９;＃共同第一作者;?通讯联系 人,EＧmail:sztang＠yzu．edu．cn) IdentifyingtheGeneticBasisofSpikeletFertilityDifferencesBetweenBTＧtype ThreeＧLinejaponicaHybrids ZHANGHongＧgen＃,QIANKai＃,SUNYiＧbiao,PEIYan,FENGZhiＧqiang,LIUXiaoＧyu,TANGShuＧzhu?, LIANGGuoＧhua,GUMingＧhong (KeyLaboratoryofCropGeneticsandPhysiologyofJiangsuProvince/KeyLaboratoryofPlantFunctionalGenomics,Ministryof Education,YangzhouUniversity,Yangzhou２２５００９,China;＃Theseauthorscontributedequallytothispaper;?Corresponding author,EＧmail:sztang＠yzu．edu．cn) ZHANGHonggen,QIANKai,SUNYibiao,etal．Identifyingthegeneticbasisofspikeletfertilitydifferencesbetween BTＧtypethreeＧlinejaponicahybrids．ChinJRiceSci,２０１４,２８(４):３７７Ｇ３８３． Abstract:InordertounderstandthegeneticbasisofspikeletfertilitydifferencesbetweenBTＧtypethreeＧlinejaponica hybrids,theCMSlinesYujing６A,９２０１Aandtheircorrespondingmaintainerlines,andtherestorerlinesMin１２０, TR２６０４wereusedtodevelopthegeneticpopulationsforstudyingthegeneticcharacteristicsofBTＧtypeCMSin differenthybridcombinations．Accordingtofertilitysegregationoftheplantsfromthepopulationsof９２０１A//９２０１B/ Min１２０,Yujing６A//Yujing６B/Min１２０and９２０１A//９２０１B/TR２６０４,itwasconcludedthatthefertilityrestoration of９２０１AandYujing６Awascontrolledbyonefertilityrestoration(Rf)gene．Then,theRfgenesinMin１２０,TR２６０４ weremappedbetwee１８９６７９９６bpwiththephysicaldistanceof１９５kban１８９６７９９６bp withthephysicaldistanceof１５０kbonchromosome１０,respectively,indicatingthattheRfgenesinMin１２０, TR２６０４mightbeRf１aaccordingtothemappingresultsofRf１informerstudies．ThefertilityrestorationofYujing ６AinthepopulationderivedfromYujing６AandTR２６０４wasalsocontrolledbyRf１aidentifiedbythegenotypeand phenotypeofplantsinYujing６A/TR２６０４//TR２６０４BC１F２population,sothesterilityofYujing６A/TR２６０４was inducedbyhybridincompatibilitybetweenthetwoparents． Keywords:threeＧlinejaponicahybrid;spikeletfertilitydifferences;fertilityrestorationgene;mapping 张宏根,钱凯,孙一标,等．BT型三系杂交