7SCMRP基因原核表达产物溶解形式分析.PPTVIP

基因原核表达系统 转录载体 翻译载体 LIC （连接非依赖的克隆） 图3.5 SCMRP基因原核表达 M：蛋白质分子量标准；泳道1：IPTG诱导BL21(DE3 pET-32b)总蛋白；泳道2~4：IPTG诱导BL21(DE3 pET-32bS)总蛋白。 Fig. 3.5 Prokaryotic expression of gene SCMRP M: Protein Molecular Weight Marker; lane1: the whole cell protein of BL21(DE3 pET-32b) induced by IPTG; lane 2~4: the whole cell protein of BL21(DE3 pET-32bS) induced by IPTG. 图3.6 SCMRP融合蛋白大肠杆菌中表达的SDS电泳图 M：蛋白质分子量标准；1、4、7泳道分别是IPTG诱导pET32b表达2、4、6小时的DE3的总蛋白；2、5、8泳道分别是IPTG诱导重组质粒pET32bS表达2、4、6小时的DE3的总蛋白；3、6、9泳道分别是无IPTG诱导时，重组质粒pET32bS表达2、4、6小时的DE3的总蛋白。 Fig.3.6 SDSresult of SCMRP recombinant protein in E. coli strain DE3. The results showed the fused proteins are expressed in E. coli by IPTG induction. The fused protein has a molecular mass of about 20KD. The samples were collected 2h, 4h, 6h after induction by IPTG from pET32b (lane1,4,7) and from pET32b-SCMRP (lane2,5,8), respectively. The samples were collected 2h, 4h, 6h from pET32b-SCMRP (lane3,6,9), respectively. M：Protein Molecular Weight Marker. 图3.7 SCMRP基因原核表达产物溶解形式分析 M：蛋白质分子量标准；泳道1、2：BL21(DE3 pET-32bS)表达蛋白质的可溶组分；泳道3：BL21(DE3 pET-32b)表达蛋白质的可溶组分；泳道5、6、7：BL21(DE3 pET-32bS)表达蛋白质的不溶组分；泳道8：BL21(DE3 pET-32b)表达蛋白质的不溶组分。 Fig. 3.7 The dissolvability analysis of prokaryotic expression product of the gene SCMRP M: protein marker; lane1-2: dissolvable group of fusion protein by BL21(DE3 pET-32bS); lane3: dissolvable group of fusion protein by BL21(DE3 pET-32b); lane5-7: insolvable group of fusion protein by BL21(DE3 pET-32bS); lane8: insolvable group of fusion protein by BL21(DE3 pET-32b). 图3.8 SCMRP原核表达产物Western blot分析 M：蛋白质分子量标准；泳道1：Ni-NTA纯化SCMRP；泳道2：IPTG诱导BL21(DE3 pET-32b)总蛋白；泳道3：IPTG诱导BL21(DE3 pET-32bS)总蛋白；泳道4：无IPTG诱导BL21(DE3 pET-32bS)总蛋白。 Fig. 3.8 Western blot analysis of prokaryotic expression product of SCMRP M: protein marker; lane1: purification protein through Ni-NTA; lane2: the whole cell protein expressed by BL21(DE3 pET-32b) induced by IPTG; lane3: the whole cell protein expressed by BL21(DE3 pET-32bS) induced by IPTG; lane4: the whole