培养基清除结皮对蛹虫草原基形成的影响.PDFVIP

菌物学报 jwxt@ 15 September 2013, 32(5): 831-837 Http:// Mycosystema ISSN1672-6472 CN11-5180/Q © 2013 IMCAS, all rights reserved. 培养基清除结皮对蛹虫草原基形成的影响 1 1 2* 1 1 1 1 李再新 张智 刘达玉 罗荣珍 赵志平 陈欲云 刘章琴 1 四川理工学院化学与制药工程学院 四川 自贡 643000 2 成都大学生物产业学院 四川 成都 610106 摘 要：为探讨培养基清除结皮对蛹虫草原基的形成影响，提高食用真菌蛹虫草的培养产量和品质，在无菌条件下， 用无菌刀切除长满菌丝的培养基上表面部分，在温差和光周期刺激下培养，考察菌丝扭结和子实体生长情况，并检 测蛹虫草子实体虫草素、虫草酸和多糖含量。结果显示培养基清除结皮组蛹虫草子实体生长快、颜色较深、易形成 孢子头，其虫草素和腺苷含量显著升高（P0.05 ），但虫草多糖含量变化不显著（P0.05 ）。培养基清除结皮可显著地 促进蛹虫草原基的形成，提高子实体产量和品质。 关键词：蛹虫草，培养基表面结皮，子实体，原基分化 Effects of medium callus on Cordyceps militaris primordium formation 1 1 2* 1 1 LI Zai-Xin ZHANG Zhi LIU Da-Yu LUO Rong-Zhen ZHAO Zhi-Ping 1 1 CHEN Yu-Yun LIU Zhang-Qin 1School of Chemical and Pharmaceutical Engineering, Sichuan University of Science and Engineering, Zigong, Sichuan 643000, China 2Faculty of Biotechnology Industry, Chengdu University, Chengdu, Sichuan 610106, China Abstract: To study the influences of medium callus on the formation of Cordyceps militaris primordium, the callose surface part of the culture was removed with a sterile knife on the sterile conditions. After removing the surface callus, the culture was incubated under the stimulation of temperature difference and photoperiod in order to further investigate the growth states of mycelium kinks and fruiting bodies, and the content changes of cordycepin, cordyceps acid and polysaccharide. Our results showed that after removing the callus the culture demonstrated significantly faster growth, darker color, more easily forming the spore head and increasing contents of cordycepin and