一种新型大鼠固定装置的制作与使用方法-中国试验动物学报.DOCVIP

设施设备 一种新型大鼠固定装置的制作与使用方法 郭洁汝，朱 雯，李承昊，尹 飞，张光伟，陶 璨，周 艺 (第三军医大学学员旅14营，重庆 400038) 【摘要】目的 制作一种新型的清醒大鼠固定装置，并规范其使用方法，以弥补现有固定方法的不足。确保无论使用者有无经验均能高效完成清醒动物固定及后续操作。 方法 用透明塑料薄膜，塑料封口机，海绵等材料设计制作大鼠固定装置，利用大鼠的逃匿天性，达到自束缚的效果。并通过固定与注射实验比较该新型方法较传统方法的优点。结果 与传统的固定方法相比，使用该新型固定装置不仅更节约时间，其中固定操作平均耗时由18.13 s减少到10.03 s,，提高了44.7；固定与注射平均耗时从139.33 s减少到52.26 s，提高了55.3，并能显著降低大鼠应激反应。 结论 该装置和方法适用于清醒大鼠固定及注射，并能由单人完成尾静脉注射和采血操作，可显著减少操作【中图分类号】R33【文献标识码】A【文章编号】1671-7856（201）-0000-00 Doi:10.3969/j.issn.1671-7856.2015.008.000 Construction and application of a new rat-holding device GUO Jie-ru, ZHU Wen, LI Cheng-hao, YIN Fei，ZHANG Guangwei, TAO Can, Zhou Yi （14th Battalion, Cadet Brigade, The Third Military Medical University, Chongqing 400038, China） 【Abstract】 Objective To provide a practical device and protocol to hold conscious rats for subsequent operations which can overcome the disadvantages of existing methods. Users can complete the experiment more efficiently, with or without prior experience. Methods Using transparent plastic film, plastic sealing machine and sponge to make a simple device for holding rats, by taking advantage of their escaping nature. To compare the performance of the new method and existing methods for holding and injecting rats. Results Compared with existing methods, the new device and method can reduce the time-consuming to hold rat by 44.7%, from 18.13 seconds to 10.03 seconds. For holding and injecting, the new method can reduce the time-consuming by 55.3%, from 139.33 seconds to 52.26 seconds. Conclusions The new device and method is good for holding and injecting rats or drawing blood from the caudal veins. It can shorten the time of operation and reduce the stress reaction in the animals. Its especially helpful for inexperienced experimenters such as students in teaching and research tasks. 【Key words】 Awaking rat; Holding device; Animal experiment 2材料和方法 2.1 固定装置的制作 2.1.1 材料 透明薄塑料膜，塑料封口机，海绵等 2.1.2 方法 按下图1A所示裁剪底边为48 c