(精编)【医学课件大全】1型糖尿病候选基因的筛选 (41p).pptVIP

1型糖尿病候选基因的筛选;1型糖尿病;Insulin-dependent-diabetes;Insulin pump;候选基因筛选;小鼠染色体上的Idd 基因座;/page/;;;;NOD小鼠的病理进程;实验设计;基因表达分析;分离纯化RNA,分析纯度 cRNA扩增及荧光标记 Agilent 41K whole mouse genome (60-mer) oligo microarray slide. Hybridization Feature Extraction Agilent Two-Color Microarray-Based Gene Expression Analysis protocol 数据分析 Log Ratio NOD/NOD.B10;;SystematicName;不同时间点表达发生变化的基因数;Figure 1: Pancreatic lymph nodes (PLN), spleen (SPL) and peripheral blood cells (PBC) from female NOD mice at 10 days, 4 weeks, 8 weeks, 12 weeks, 16 weeks, or 20 weeks of age were processed for microarray mRNA analysis of gene expression. Diagrams indicates the numbers of milestone genes in PLN, SPL and PBC in each group (time point) according to SI Table 2. Non-fasting serum glucose of the mice used for data analysis (Data are expressed as Mean ± SD). ;;Figure 2: Venn diagram showing numbers of significantly dysregulated genes shared by the three tissues (PLN, SPL and PBC) from 10 days to 4 weeks (A; early stage) and from 8 to 20 weeks of age (B; late stage).;Figure 5: Hierarchic clustering analysis for all milestone gene expression profiles indicates the five distinct clusters in PLN while two simple clusters were detected in both PBC and SPL.;;基因表达变化;N pattern in PLN;PLN中“N”型基因;;Deaf1;SAND结构域;蛋白－蛋白相互作用结构域;DEAF1: a ‘N’ Pattern Gene;1 ggcacgagag tccgt atgga ggactcggac tcggcggcaa aacagctggg cctggccgag 61 gcggcagctg tggcggccgc tgccgcggtg gcggcggccg ccgcggccgc agcggaaagc 121 gaggcggagg agccggtgtt aagcagagac gaggactcgg aggaggacgc agactccgag 181 gcggagcgcg agacgcggcg ggtcaccgct gtggcggtga tggcggccga atctgggcac 241 atggatatgg gtaccgaggc tctgcccagc cccgatgagg ccgccgccgc cgccgccgcc 301 ttcgcagaag tgaccacagt gactgtggcc aacgtggggt cctctgcaga taacgtcttc 361 acaacatcag tggcaaacgc agcatcgata tcaggacatg tcctgtctgg taggacagcc 421 ctgcagatcg gtgacagcct gaacactgaa aaagccacac taatagttgt acacacagat 481 gggagcattg tggagaccac tgggctgaag ggcccagcag cacctctcac tccaggtccc 541 cagtctcctc ctaccccact ggcacctggc caggagaaag gtggta