昆明小鼠经寰枢椎蛛网膜下腔置管法的建立-中国试验动物学报.DOC

小鼠经寰枢椎蛛网膜下腔置管法的建立 陈锐，王华，江伟 （上海交通大学附属第六人民医院麻醉科，上海 200233） 【摘要】目的 介绍一种新型小鼠蛛网膜下腔置管法，并与传统的Wu方法相比较，为脊髓水平的药物生理研究及疼痛治疗提供更有效方法。方法 选择40只24-28g雄性昆明小鼠，随机分为Wu组和寰枢椎（AA）组，分别从腰5-6椎间隙、寰枢椎间隙置管至蛛网膜下腔。模型建立后，观察各组小鼠置管后死亡率、肢体瘫痪率，并测定其缩足反应阈值（50%PWT）、甩尾反应潜伏期（TF）和吗啡作用。对比两组是否有差异。结果 置管后Wu组和AA组在死亡率无差异的情况下，AA组的肢体瘫痪率明显低于Wu组；术后7d内，AA组大鼠的50%PWT值和TF值与术前基础值相比最为接近；吗啡镇痛及耐受实验说明此新方法可以进行小鼠长期给药。结论 小鼠经寰枢椎蛛网膜下腔置管方法具有瘫痪率低、恢复快、术后并发症少等优点，是一种新型有效的小鼠鞘内置管法。 【关键词】 寰枢椎;鞘内置管;动物模型; 【基金项目】国家自然科学基金—青年支持（编号 2【作者简介】陈锐（1988—），女，研究方向：疼痛相关基础研究。E-mail:cryourfriend@163.com 3【通讯作者】江伟（1961—），男，研究方向：麻醉药理学及疼痛相关基础研究。E-mail:jiangw@ Chronic atlantoaxial catheterization of the spinal subarachnoid space in mice CHEN Rui, WANG Hua, JIANG Wei (Department of Anesthesiology, the Sixth People’s Hospital, Shanghai Jiaotong University School Of Medicine, Shanghai 200233, China) 【Abstract】Objective To establish a new catheterization of the spinal subarachnoid space in mouse and to compare with the traditional catheterization, which can be used for the research on neurophysiology and pain treatment. Methods forty male Kunming mice weighing 24-28g were randomly divided into two groups: Wu group and axis-atlantoaxial group (AA group), respectively catheter from the intervertebral space of lumbar 5-6 and atlantoaxial to the subarachnoid space. After the models were established, we observed the percentage of death and paralysis of limbs, and also measured 50%paw withdrawal threshold (50%PWT), tail flick latency and analgesic effect of morphine to compare the difference of two groups. Results In the case of the same death rate in Wu group and AA group, the percentage of paralysis of limbs in AA group was obviously lower than Wu group. Within 7 days of catheterization, 50%PWT and the TF of AA group was closest to baseline. Morphine analgesic experiment showed the new method adapt to long-term injection. Conclusion The atlantoaxial catheterization model of the spinal subarachnoid space is characterized by it