不同致病型马立克氏病病毒8kb基因家族序列比较3.pdf

不同致病型马立克氏病病毒 1. 8kb 基因家族序列比较 1 1 1 1 2 张纪元 ,丁家波 ,崔治中 ,姜世金 , Bublot Michel ( 1. 山东农业大学动物科技学院 , 山东泰安 ,27 10 18 ; 2 . Merial Select , Inc , 29 Ave . Dony , Garnier L yon , 69007 , France) Comparison of 1. 8kb gene family of Marek′s Disease Viruses of Different Pathotypes ZHAN G J iyuan1 , D IN G J iabo1 , CU I Zhizhon g1 ,J IAN G Shij in1 , Bublot Michel2 ( 1. Col leg e of A ni m al S cience an d Technology , S hang dong A g ricul t u ral Uni vers i ty , Tai’an 27 10 18 , Chi na; 2 . M eri al S elect , ) I nc , 29 A ve. D ony , Garnier L y on , 69007 , F rance Abstract : In t hi s st udy , sequence s of t he 1. 8kb gene f amily of 12 diff erent st rain s of Marek ′s di s ea se vivu se s were co mp ared . The se st rain s covered 4 different p at hot yp e s , i . e , vaccine , virulent , very vir ulent , and very vir ulent p lu s st rain s , p lu s t hree fiel d st rain s i solat ed in China . The re sult s demon st rat ed t hat t he ho molo gy of t he up st ream regulating sequence s of t he gene f amily wa s more t han 92 . 5 % amon g 12 st rain s , vaccine st rain CV I988/ Ri sp en s had more mut ation s e sp ecial ly wit h a “C TC GG deletion bet ween TA TA bo x and a SP 1 enhancer . There were 37 con secutive rep eat s of 132 bp in t he 1. 8 kb gene f amily of vaccine st rain CV I988/ Ri sp en s , but only 2 cop ie s were det ect ed in t he left st rain s of all p at hot yp e s. However , t here were al so only 2 copie s of 132 bp rep eat s found even in Chine se vaccine st rain 814 , in dicatin g t hat exp a