输血实验 Microsoft PowerPoint 演示文稿.讲解材料.pptVIP

TEST PROCEDURE 反定型： 取小试管三只，分别用记号笔标记Ac、Bc、Oc后，加受检者血清一滴，然后加标准A、B及O型RBC各一滴。 reverse grouping Add one drop of serum/plasma to three tubes separately Add one drop of 5% standard RBC to three tubes separately TEST PROCEDURE ? 将上述混匀后静置数分钟或3000r/m离心15s肉眼判断凝集度。 Shake gently to mix contents of reaction tubes. Centrifuge six tubes with 3000r/s,15m. Shake gently with 45-60 angles. Evaluate six tubes for agglutination and/or hemolysis upon test completion. Record the reaction strength on the paper. 4+ 一个结实的大凝块，背景清楚。 3+ 数个结实的大凝块，背景清楚。 2+ 中等大小的凝块加小凝块，背景清楚。 1+ 小凝块，背景较浑浊。 +或W+ 细胞有微小凝块，背景红色浑浊，通常用显微镜