植物维生素E合成相关酶基因的克隆及其在体内功能研究进展Progress in ....pdfVIP

植物学通报 2006, 23 (1): 68~77 Chinese Bulletin of Botany . . 专题介绍 植物维生素E合成相关酶基因的克隆及其在体内功能研究进展 潘卫东 李晓峰 陈双燕 刘公社* ( 中国科学院植物研究所光合作用与环境分子生理学重点实验室 北京 100093) 摘要 维生素E(VE) 的天然产物有8种类型, 分别为、、 、-生育酚(tocopherol)和、、 、-生育三 烯酚(tocotrienol), 对植物、动物和人类都具有十分重要的生理作用。医学证明, 维生素E不仅与生殖系统, 而且与中枢神经系统、消化系统、心血管系统和肌肉系统的正常代谢都有密切关系; 它也是治疗冠心 病、动脉粥样硬化、贫血、脑软化、肝病和癌症等的辅助药物。而绿色植物则是人类和动物VE 的基本 来源。近年来随着植物基因组学的发展和营养基因组学概念的提出, 通过快速分离植物营养代谢(VE)相 关酶的基因, 最终解析和调控植物微量营养素代谢途径, 利用代谢工程的方法大大提高植物营养价值之 策略正在逐步完善。本文对有关植物中VE生物合成途径和相关酶基因克隆研究现状, 以及VE在植物体 内的作用和功能研究进展进行了综述, 以期为VE作用机理的探寻和功能开发提供新思路。 关键词 代谢途径, 维生素E, 基因功能, 代谢工程 Progress in Vitamin E Synthesis-related Enzyme Genes and in vivo Functions in Plants * Weidong Pan, Xiaofeng Li, Shuangyan Chen, Gongshe Liu (Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences , Beijing 100093) Abstract There are 8 natural products of vitamin E designated as , , , -tocopherol and , , , - tocotrienol. Vitamin E plays an important role in plants, animals and humans physiologically. Vitamin E has close relations not only with reproduction but also with the central nervous system, digestive system, cardiovascular system and muscles. Also, it can be used as drug therapy for coronary disease, anaemia, and carcinoma. Green plants are basic sources of vitamin E for humans and animals. With the development of plant genomics and tropical genomics by rapid isolation of plant tropic metabolism-related enzyme genes and resolving the metabolism pathways, we are gradually ap- proaching a strategy aimed at a better trophic value plus plant resources using metabolic engineering. In this context, this article discusses the current progress in vitamin E synthesis-related enzyme gene cloning and in vivo function expl