现代光分析-6 标记技巧方法.pptVIP

第四章 荧光法在生物分析中的应用;Fluorescence Probes in Bioanalysis ;蛋白质、核酸、多糖的荧光标记;Fluorescein;例三;一、蛋白质的活性功能团;利用蛋白质的活性功能团对蛋白质进行标记;1. Amine-Reactive Probes fluorescein-5-isothiocyanate (FITC Isomer I) ; fluorescein-6-isothiocyanate (FITC Isomer II) ; Oregon Green? 488 carboxylic acid ; Rhodamine Red?-X, succinimidyl ester ; 4-dimethylaminoazobenzene-4-sulfonyl chloride (dabsyl chloride) ; Texas Red? sulfonyl chloride ;Spectra — Texas Red-BSA conjugate Absorption and fluorescence emission spectra of the Texas Red conjugate of bovine serum albumin (A-23017) in pH 7.0 buffer. ????????????????????????????????