抗生素(英文精品) Antimicrobial activity of Nigella sativa oil against multi-drug resistant Pseudomonas aerugenosa from clinical specimens重点培训.pptVIP

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2018-04-02 发布于天津
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抗生素(英文精品) Antimicrobial activity of Nigella sativa oil against multi-drug resistant Pseudomonas aerugenosa from clinical specimens重点培训.ppt

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Antimicrobial activity of Nigella sativa oil against multi-drug resistant Pseudomonas aerugenosa from clinical specimens. ? Mohd. Tariq Salman, Rahat Ali Khan and Indu Shukla* Departments of Pharmacology and Microbiology* Jawaharlal Nehru Medical College Introduction Traditional healers have long used plants to prevent/cure infective conditions. Nigella sativa L. (Kalonji) has been used for centuries for treatment of various ailments, including infections. Its seeds, commonly used in recipes in Asian countries are reported to possess many medicinal properties. Its crude extracts, oil possess antibacterial activity against many pathogenic bacteria. Objective: To study effect of N. sativa essential oil against standard and multidrug resistant strains of Pseudomonas aerugenosa. Materials and Methods N. Sativa oil (Mohammedia products, Hyderabad) tested in various dilutions by disc agar diffusion technique using impregnated filter paper discs on inoculated Muellar Hinton agar plates against P. Aerugenosa (ATCC 27853 and NCTC 10662) and 22 clinical isolates of pseudomonas obtained from pus, blood, ear discharge and conjunctival swab of patients attending J.N.M.C. Hospital, Aligarh during 2003 2004. Out of the clinical isolates taken, 3 were resistant to 2-4 antibiotics, 6 to 5-7 antibiotics, 8 to 8-10 antibiotics and 5 to 11-13 antibiotics. Resistance was highest for Ampicillin, Gentamicin, Ciprofloxacin, Amikacin, Ceftazidime, Cefotaxime, Ofloxacin and Ceftriaxone. Observations Effect of N. sativa oil against standard strains. (Mean diameters of zones of inhibition in mm around 6 mm discs impregnated with Oil or commercial diagnostic antibiotic sensitivity testing disc) Conc./disc Diameters of zones of inhibition in mm P. aerugenosa (NCTC 10662) P. aerugenosa (ATCC 27853) 1:1 25 24 1:10 20 20 1:50 19 20 1:100 12 - Ethylene glycol - - Ampicillin (10μg/disc) 16 - ResultsActivity of N. sativa oil was seen: upto a dilution