抗生素(英文精品) Metabolic engineering of bacteria资料汇总.pptVIP

Metabolic engineering of bacteria;Many biological small molecules are useful;Increasing production of antibiotics (and other small molecules)--traditional methods;An alternative to simple random mutagenesis: genome shuffling;;Test case: increase tylosin production by S. fradiae?;Comparing CSI to genome shuffling;Genome shuffling;Increasing production of a biological compound: rational design;Engineering E. coli to produce indigo Mutate tryptophan synthase complex to release indole Introduce napthalene dioxygenase (from Pseudomonas putida);Introduce isatin hydrolase (from a soil microbe) to prevent production of indirubin (color) from isatin;Potential routes for overproducing biological compounds ;How to overproduce phenylalanine?;Rational metabolic engineering;Non-E.coli Bacterial Cloning Homologous recombination ;Cloning in bacteria other than E.coli?;Necessary components for non-E.coli cloning;Natural transformation;Conjugation as a method of transfer;Plasmid Host Range;Integration by recombination;Homologous recombination: portrait of a single cross-over;Recombination (single crossover);Recombination in genome engineering:;Things that can be easily done with PCR products, transformation, and recombination….;Recap