抗生素的新型用法(英文教学)Novel Approach for Improving the Productivity of Antibiotic-Producing Strains by Inducing Combined Resistant Mutations总结概括.pptVIP

Novel Approach for Improving the Productivity of Antibiotic-Producing Strains by Inducing Combined Resistant Mutations ;INTRODUCTION;Actinorhodin- a blue pigmented polyketide antibiotics;certain mutations conferring streptomycin resistance give rise to secondary metabolite production (by an unknown mechanism) without the requirement for ppGpp in Streptomyces lividans and S.?coelicolor A3(J.Bacteriol,1999:6958-6968). Acquisition of resistance to other aminoglycoside antibiotics, such as gentamicin and Geneticin, also confers the ability to produce actinorhodin in S.?lividans 66,?which normally does not produce actinorhodin (H.?Hu and K.?Ochi, unpublished). Furthermore, by inducing mutations conferring resistance to rifampin, an ansamycin antibiotic, we were able to restore the impaired actinorhodin production in the relA and relC mutants of S.?coelicolor A3(2) (Y.?Tozawa, J.?Xu, and K.?Ochi, unpublished data). So the present study aims to develop novel approaches for improving antibiotic-producing strains, especially focusing on methods to induce combined drug-resistant mutations. ;Summary of mutations on the S.?coelicolor ;Mutants and colony morphology;;;;FIG. 5. ? Western blotting analysis of the ActII-ORF4 protein, a pathway-specific positive regulator in the actinorhodin biosynthesis pathway. ;Summary;PROSPECTS;