马传染性贫血病毒基因非编码区ltr嵌合克隆的构建3.pdfVIP

马传染性贫血病毒基因非编码区L TR 嵌合克隆的构建 1 ,2 1 2 3 3 2 3 魏丽丽 ,王晓钧 ,王 　盈 ,粱 　华 ,沈 　韬 ,李景鹏 ,张晓燕 , 1 1 1 相文华 ,邵一鸣 ,沈荣显 ( 1. 中国农业科学院哈尔滨兽医研究所 ,黑龙江哈尔滨 15000 1 ; 2 . 东北农业大学生命科学院 ,黑龙江哈尔滨 150030 ; 3 . 中国疾病 预防控制中心 性病艾滋病预防控制中心 ,北京 100050) Construction of a Chimeric Inf ectious Clone of Chinese Equine Inf ectious Anemia Virus by Partial L TR Substitution W EI Lili1 ,2 , WAN G Xiaoj un1 , WAN G Ying2 , L IAN G Hua3 , SH EN Tao3 , L I J in gp eng2 , Zhang Xiaoyan3 , XIAN G Wenhua1 ,SHA O Yiming3 , SH EN Rongxian1 ( 1. H arbi n Veteri nary R esearch I ns t it ute of CA A S , H arbi n 15000 1 , Chi na; 2 . N ort heas t A g ricul t u ral Uni vers ity , Col leg e of L if e S ciences , H arbi n 150030 ; 3. N at ional Center f or A I D S / S TD P revent ion an d Cont rol , Chi na CD C , B eij i ng 100050) Abstract : Accor ding to mut ation s occur red in t he p a ssage s during t he Equine infectiou s anemia vi r u s ( EIA V) vaccine at t enuation , a f ull lengt h chimeric gene clone , pL GFD912 , wa s con st r uct ed succe ssf ully at a backbone of clone pL GFD38 by sub stit ution wit h L TR U 3 region of a vir ulent EIA V st rain . The pL GFD912 wa s u sed to t ran sf ect fet al do nkey der mal ( FDD) cell s an d p a s saged in donkey leukocyt e (DL ) cult ure . The cell cult ure s were monitored by R TPCR , rever se t ran scrip t a se activit y a ssay and realtime R TPCR . The re sult s of R T activit y and R TPCR were po sitive in t he sup er nat ant of cell cult ure s a