监测ABO不相容性肾移植的【精品-ppt】.ppt

* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * Plasmapheresis Procedures ABO Antibody Titer (AHG) Pretransplant Treatments (#) Posttransplant Treatments (#) 16 2 2 16 2 2 32 3 3 64 4 3 128 5-6 3 256 7-8 4 512 9-10 4 1024 10-12 4 1024 15 5 * 血浆置换过程 ABO抗体滴度 移植前治疗次数 移植后治疗次数 16 2 2 16 2 2 32 3 3 64 4 3 128 5-6 3 256 7-8 4 512 9-10 4 1024 10-12 4 1024 15 5 * ABO Antibody Titers Conventional test tube method AABB technical manual 15th ed. Bethesda: American Association of Blood Banks, 2005. Serial dilutions of each sample were prepared in 0.9% saline Pooled indicator cells (Immucor, Norcross, GA) of the appropriate ABO type were added 30 minutes 22C incubation test phase 30 minutes 37C incubation test phase AHG test phase using monospecific anti-IgG (Immucor, Norcross, GA) Agglutination was scored using the Marsh 0 to 12 scoring system AHG titer endpoint was the reciprocal of the highest dilution demonstrating macropscopic (score 3) agglutination * ABO抗体滴定 常规的试管法 AABB技术手册 样品用0.9%的盐水进行连续稀释 加入适量的混合ABO血型指示细胞（ Immucor ，Norcross, GA ） 22度孵育30分钟 37度孵育30分钟 单特异性抗-IgG (Immucor, Norcross, GA) 的抗人球蛋白（AHG）实验阶段 用Marsh 0到12打分系统给凝集打分 AHG最终滴度是样品出现肉眼可见的（3分）凝集的最高稀释倍数 * ABO Antibody Titers It is time consuming Turn around time is critical Our experience indicates that only the AHG titer values are critical Titer endpoint is demonstrated with a macroscopic agglutination * ABO抗体滴度 这是一个花费时间的实验 关键是出现结果的时间 我们的经验表明只有AHG滴度是关键值 最终滴度是由肉眼可见的凝集决定 * Objectives The goal of the present study was to streamline ABO antibody titer determinations by assessing AHG titers using 1) A revised test tube method where the room temperature incubation phase was eliminated 2) An anti-IgG gel microcolumn method * 目 的 本研究的目的是通过评估AHG 滴度，系列测定ABO抗体 1) 应用不需要室温孵育过程的改良试管法 2) 应用抗-IgG凝胶微柱法 * Ma